Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model  

作　　者：Yan-Kun Yue Xiao-Li Chen Shan Liu Wu Liu 

机构地区：[1]Department of Ophthalmology,Fuxing Hospital,Capital Medical University,Beijing 100038,China [2]Department of Ophthalmology,Beijing TongRen Hospital,Capital Medical University,Beijing 100730,China

出　　处：《International Journal of Ophthalmology(English edition)》2021年第12期1813-1819,共7页国际眼科杂志（英文版）

基　　金：National Natural Science Foundation of China(No.81800827).

摘　　要：AIM:To investigate whether upregulation of apoptosisstimulating p53 protein 2(ASPP2)expression could alleviate the development of proliferative vitreoretinopathy(PVR)in a rat model.METHODS:ASPP2-lentivirus or scrambled-lentivirus were transfected into ARPE-19 cells,followed with measurements of cell cytotoxicity by cell counting kit-8 assay.ASPP2 upregulation was confirmed by Western blotting and immunocytochemistry.Then ARPE-19 cells pretreated with ASPP2-lentivirus were intravitreally injected to Brown Norway rats to induce PVR models.PVR development and retinal function were evaluated by retinal photography and electroretinography,respectively.Finally,epithelial-mesenchymal transition as well as autophagy were investigated in rats’retinas via Western blotting.RESULTS:Protein expression of ASPP2 was significantly upregulated by ASPP2-lentivirus transfection in ARPE-19 cells.The development and progression of PVR were impeded significantly in rats with intravitreal injection of ARPE-19 cells pretreated with ASPP2-lentivirus.Accordingly,retinal functions were less affected and PVR grades were much lower in rats with ASPP2-lentivirus compared to scrambledlentivirus treatment.Moreover,epithelial-mesenchymal transition and autophagy markers were decreased in the retinas of rats treated with ASPP2-lentivirus.CONCLUSION:ASPP2-lentivirus transfected to ARPE-19 cells mitigates the progression of PVR in rat models,which might be partly through reduced autophagy and attenuated epithelial-mesenchymal transition.ASPP2 might stand as a new approach for PVR treatment in the future.

关 键 词：proliferative vitreoretinopathy apoptosisstimulating p53 protein 2 epithelial–mesenchymal transition AUTOPHAGY ARPE-19 

分 类 号：R774.1[医药卫生—眼科]