ARTP诱变法提高蜡状芽孢杆菌中壳聚糖酶的产量  

ARTP Mutagenesis Improves the Production of Chitosanase in Bacillus cereus

作　　者：张朝正[1] 李意 赵华[1] ZHANG Chaozheng;LI Yi;ZHAO Hua(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《食品工业科技》2022年第1期141-146,共6页Science and Technology of Food Industry

摘　　要：利用常温常压等离子体诱变(Atmospheric and Room Temperature Plasma, ARTP)蜡状芽孢杆菌,筛选获得酶活提升的菌株。结果表明,蜡状芽孢杆菌在种子液中培养6 h可达到对数期中后期,常温常压等离子体诱变时间为60 s时,蜡状芽孢杆菌的致死率达到85%,筛选得到一株壳聚糖酶活性提高13.19%的突变株,然后进行酶活稳定性验证,经六代培养的平均酶活为9.643367 U/mL,波动在5%之内,表明其产酶量高,稳定性强,可用于后续出发菌株。诱变后菌株经电镜观察,发现菌落形态无明显变化,但是菌株的个体形态发生了变化,比原始菌株细长,证明酶活提高是ARTP诱变起到的作用。Atmospheric and room temperature plasma(ARTP) Bacillus cereus was used to screen the strains with increased enzyme activity. The results showed that culturing Bacillus cereus in the seed solution for 6 hours could reach the middle and late logarithmic phase. When the mutagenesis time of normal temperature and atmospheric pressure plasma was 60 s,the lethality of Bacillus cereus reached 85%. A mutant strain with a 13.19% increase in chitosanase activity was screened.Then the stability of the enzyme activity was verified. The average enzyme activity after six generations of culture was9.643367 U/mL, and the fluctuation was within 5%, indicating that its enzyme production was high and the stability was strong, and it could be used for subsequent starting strains. After the mutagenesis, the strain was observed by electron microscope, and it was found that the morphology of the colony did not change significantly, but the individual morphology of the strain had changed, and it was slenderer than the original strain, which proved that the increase in enzyme activity was the effect of ARTP mutagenesis.

关 键 词：壳寡糖 壳聚糖酶 常温常压等离子诱变 酶活 蜡状芽孢杆菌 

分 类 号：TS202.3[轻工技术与工程—食品科学]