Fibroblast Growth Factor 19 Induced Changes in Non-malignant Cholangiocytes  

作　　者：Jiaqi Yang David Sontag Sam Kung Gerald Y.Minuk 

机构地区：[1]Section of Hepatology,Department of Medicine,Rady College of Medicine,University of Manitoba,Winnipeg,Manitoba,Canada [2]Department of Immunology,Rady College of Medicine,University of Manitoba,Winnipeg,Manitoba,Canada [3]Department of Pharmacology and Therapeutics,Rady College of Medicine,University of Manitoba,Winnipeg,Manitoba,Canada

出　　处：《Journal of Clinical and Translational Hepatology》2021年第6期909-916,共8页临床与转化肝病杂志（英文版）

摘　　要：Background and Aims:Fibroblast growth factor(FGF)19 has been implicated in the pathogenesis of murine hepatocellular carcinoma.Whether it plays a role in the development or course of human cholangiocarcinoma remains to be determined.The aim of this study was to determine whether prolonged exposure to FGF19 results in the transformation of non-malignant human cholangiocytes into cells with malignant features.Methods:Human SV-40 transfected non-malignant H69 cholangiocytes were cultured with FGF19(0-50 ng/mL)for 6 weeks,followed by 6 weeks with medium alone.Cell proliferation,invasion,stem cell surface markers,oncofetoprotein expression,state of differentiation,epithelial-mesenchymal transition(EMT)and interleukin(IL)-6 expression were documented at various time intervals throughout the 12-week period.Results:FGF19 exposure was associated with significant increases in cell proliferation,de-differentiation,EMT and IL-6 expression.However,each of these effects returned to baseline or control values during the 6-week FGF19 free follow-up period.The remaining cell properties remained unaltered.Conclusions:Six weeks of FGF19 exposure did not result in the acquisition of permanent malignant features in non-malignant,human cholangiocytes.

关 键 词：Fibroblast growth factor 19 CHOLANGIOCYTES CHOLANGIOCARCINOMA Farnisoid x receptor Obetcholic acid 

分 类 号：R73[医药卫生—肿瘤]