保健食品中花色苷分析方法研究  

Study on Analytical Method of Anthocyanin in Health Food

作　　者：柏伟荣[1] 张书嘉[1] 郭小莉[1] 高如意[1] BAI Wei-rong;ZHANG Shu-jia;GUO Xiao-li;GAO Ru-yi(Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang,Jiangsu 222001,China)

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《粮油食品科技》2022年第1期150-156,共7页Science and Technology of Cereals,Oils and Foods

摘　　要：为了建立保健食品中花色苷测定方法,采用薄层色谱法(TLC)对花色苷进行定性鉴别,采用高效液相色谱法(HPLC)对花色苷进行定量检测。结果发现,TLC鉴别的适宜条件为:展开剂各组分比例为V(甲酸)∶V(水)∶V(正丁醇)=9.1∶8.4∶32.5;点样量为2μL。HPLC检测时花色苷在4.361~52.332μg/mL(R;=0.999 5)内线性关系良好,平均加样回收率为98.22%(RSD=1.23%,n=6),且精密度、重复性、稳定性良好。In order to establish a method for the determination of anthocyanins in health foods, thin-layer chromatography(TLC) was used for qualitative identification of anthocyanins, and high-performance liquid chromatography(HPLC) was used for quantitative detection of anthocyanins. The results showed that the suitable conditions for TLC identification were developing agent: V(formic acid)∶V(water-n)∶V(butanol)=9.1∶8.4∶32.5, spotting amount: 2 μL. Anthocyanin was detected at 4.361~52.332 μg/mL(R2 =0.999 5).The internal linear relationship is good, with the average sample recovery rate is 98.22%(RSD=1.23%, n=6),and the precision, repeatability and stability are good. Experiments show that the established thin-layer identification method for anthocyanins is stable and feasible.

关 键 词：保健食品 缓解视疲劳 花色苷 薄层色谱法 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]