蚕蛹粉对红酵母发酵生产类胡萝卜素的影响  

Effects of Silkworm Pupae Powder on Carotenoid Fermentation by Rhodotorula sp.

作　　者：陈欣 王欣蕾 林兰萍 曹丽彬 张凯 谭晓锡 CHEN Xin;WANG Xin-lei;LIN Lan-ping;CAO Li-bin;ZHANG Kai;TAN Xiao-xi(School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212100,China)

机构地区：[1]江苏科技大学生物技术学院,江苏镇江212100

出　　处：《粮油食品科技》2022年第1期190-195,共6页Science and Technology of Cereals,Oils and Foods

摘　　要：研究蚕蛹粉浓度、葡萄糖浓度、培养时间以及发酵液初始p H对一株红酵母发酵生产类胡萝卜素的影响。单因素实验结果表明:添加7.5 g/L的蚕蛹粉、葡萄糖浓度为30 g/L,发酵时间4 d,发酵液初始p H 7.0时,类胡萝卜素产量达到最高值14.51 mg/L,较对照组提高46.9%;通过正交实验进一步优化培养条件,结果表明:各因素对类胡萝卜素发酵产量的影响大小顺序为:培养时间>蚕蛹粉浓度>葡萄糖浓度>培养基初始p H;从极差R可知,最佳发酵条件组合为A2B2C2D1,即蚕蛹粉浓度7.5 g/L、葡萄糖浓度30 g/L、培养时间4.0 d、培养基初始pH 6.0。经过优化,类胡萝卜素产量达到最大值14.88 mg/L。Effects of silkworm pupae powder concentration, glucose concentration, cultural time and initial pH of culture medium on the fermentation of carotenoid were investigated. The results of single-factor experiments showed that when silkworm pupae powder concentration was 7.5 g/L, with glucose concentration at 30 g/L and cultural time of 4 d, as well as initial pH of culture medium of7.0, the carotenoid yield reached the maximum value of 14.51 mg/L, which was 46.9% higher than that of the control. In order to make further improvement on the carotenoid production, the orthogonal tests were performed and the results showed that the order of the factors affecting the carotenoid yield was as follows: cultural time>silkworm pupae powder concentration>glucose concentration>p H. The carotenoid yield reached the maximum value of 14.88 mg/L under the optimum fermentation conditions. Silkworm pupae powder concentration was 7.5 g/L, with glucose concentration at 30 g/L, and cultural time of 4 d, plus pH value at 6.0.

关 键 词：蚕蛹粉 类胡萝卜素 红酵母 发酵 正交实验 

分 类 号：Q819[生物学—生物工程]