多羽凤尾蕨总黄酮提取工艺优化及抗氧化性研究  

Study on optimum extraction and antioxidative activity of total flavonoids from Pteris decrescens

作　　者：赵旭萍 杨柳 刘亚华 叶江海 何康 邹娟 ZHAO Xu-ping;YANG Liu;LIU Ya-hua;YE Jiang-hai;HE Kang;ZOU Juan(College of Traditional Chinese Medicine,University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州中医药大学中药材学院,贵州贵阳550025

出　　处：《粮食与油脂》2022年第1期129-132,138,共5页Cereals & Oils

基　　金：国家自然科学基金项目(81660723);贵州省科技计划项目[黔科合平台人才(2017)5618];贵州省一流学科项目。

摘　　要：建立以紫外-可见分光光度计法测定多羽凤尾蕨中总黄酮含量的方法。在此基础上,以总黄酮含量为评价指标,采用正交实验优化多羽凤尾蕨总黄酮提取工艺,并通过清除DPPH自由基和OH自由基方法评价多羽凤尾蕨总黄酮抗氧化性。最佳提取工艺为乙醇体积分数70%,料液比1∶20 (g/mL),提取时间50 min,提取次数3次,以该方案超声提取,药材中提取的总黄酮含量为0.72 mg/g。提取得到的多羽凤尾蕨总黄酮对DPPH自由基和OH自由基表现出良好的清除能力,且该测定方法准确可靠、简单、便捷、高效。An UV-Vis spectrophotometer was established to determine the content of total flavonoids in Pteris decrescens. On this basis,the extraction process of total flavonoids was optimized by orthogonal experiment with the content of total flavonoids as the evaluation index. The antioxidant activities of total flavonoids were evaluated by DPPH and OH radical scavenging methods. The optimal extraction conditions were as follows: volume fraction of ethanol 70 %,solid-liquid ratio 1 ∶ 20( g/mL),extraction 50 min and extraction 3 times. Extraction content of total flavonoids in the medicinal materials was 0. 72 mg/g by ultrasonic extraction scheme. The total flavonoids extracted from Pteris decrescens showed good scavenging ability on DPPH and OH free radicals,and the method was accurate,reliable,simple,convenient and efficient.

关 键 词：多羽凤尾蕨 总黄酮 提取工艺 正交设计 抗氧化性 

分 类 号：TS201.1[轻工技术与工程—食品科学]