中高温大曲中霉菌的分离及其麸曲制备  

Isolation of Mold from Medium High Temperature Daqu and Preparation of Fuqu

作　　者：郑自强 卫春会 张立伟 邓杰 黄治国 刘美君 任志强 ZHENG Ziqiang;WEI Chunhui;ZHANG Liwei;DENG Jie;HUANG Zhiguo;LIU Meijun;REN Zhiqiang(School of Biological Engineering,Sichuan University of Technology,Yibin 644000,China;Liquor Making Biotechnology and Application Key Laboratory of Sichuan Province,Yibin 644000,China;School of Chemical Engineering,Changchun University of Technology,Changchun 130000,China)

机构地区：[1]四川轻化工大学生物工程学院,四川宜宾644000 [2]酿酒生物技术及应用四川省重点实验室,四川宜宾644000 [3]长春工业大学化学工程学院,吉林长春130000

出　　处：《现代食品科技》2022年第1期165-172,共8页Modern Food Science and Technology

基　　金：四川省科技厅应用基础研究项目(2019YJ0474);中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2018JJ014);酿酒生物技术及应用四川省重点实验室开放基金项目(NJ2018-02)。

摘　　要：从中高温大曲中筛选产糖化酶菌株,并制作霉菌麸曲,对麸曲的制作条件和酿酒性能进行测定。分离得到两株高产糖化酶菌株JQ-1、JQ-2,其鉴定结果分别为米根霉菌(Rhizopusoryzae)和卷枝毛霉菌(Mucorcircinelloides)。为了使菌株能更好的应用生产,以JQ-1、JQ-2菌株制备霉菌麸曲,制曲性能测试结果表明,以全麸皮为制曲原料,控制加水量为55%,JQ-1麸曲培养96 h,JQ-2麸曲培养72 h,干燥温度为40℃,该条件利于麸曲糖化酶活力的保持,所得JQ-1麸曲糖化酶活力达1510.33 U/g,JQ-2麸曲达1388.26U/g;产酒性能测试结果表明,麸曲JQ-1所酿基酒的出酒率达44.42%,酒精度为41.22%vol,麸曲JQ-2所酿基酒的出酒率达37.32%,酒精度为35.84%vol,麸曲JQ-1的酿造性能更为突出。综合制曲和生产性能,发现菌株JQ-1和JQ-2能在酿造生产中较好的发挥其糖化作用,且酿造性能麸曲JQ-1>麸曲JQ-2。该研究以期为大曲功能微生物的开发与生产应用提供一定的指导。Strains producing glucoamylase were screened from the high temperature Daqu,used to make mould Fuqu,and its production conditions and brewing performance were determined.Two high glucoamylase producing strains JQ-1 and JQ-2 were isolated and identified as Rhizopus oryzae and Mucor circinelloides,respectively.To make the strains more suitable for production,JQ-1 and JQ-2 were used to making mould Fuqu,The test results of Fuqu making performance showed that using whole bran as Fuqu making raw material,controlling the amount(55%) of water(JQ-1 for 96 h and JQ-2 for 72 h) and drying temperature of 40 ℃,these conditions were conducive to the maintenance of glucoamylase activity of Fuqu.The glucoamylase activity of JQ-1 Fuqu and JQ-2 Fuqu reached 1510.33 U/g and 1388.26 U/g,respectively.The results of liquor production performance test showed that the liquor yield of base liquor made by bran Fuqu JQ-1 was 44.42%,and the alcohol degree was 41.22% vol;the liquor yield of base liquor made by bran Fuqu JQ-2 was 37.32%,and the alcohol degree was 35.84% vol.The brewing performance of bran Fuqu JQ-1 was more outstanding.Based on the comprehensive Fuqu preparation and production performance results,strains JQ-1 and JQ-2 were found to play a good role in saccharification in brewing production.And in terms of brewing performance,Fuqu JQ-1>Fuqu JQ-2.This study aims to provide some guidance for the development and production applications of functional microorganisms in Daqu.

关 键 词：大曲 糖化酶 霉菌 麸曲 生产性能 

分 类 号：TS261.11[轻工技术与工程—发酵工程]