β-乳球蛋白多酚三配体复合物纳米颗粒的制备与表征  

Preparation and Characterization of β-Lactoglobulin Nanoparticles Loaded with Three Polyphenols

作　　者：姬雨雪 郑丽丽[2,3] 杨旸 钟爽 艾斌凌[2,3] 郑晓燕 校导[2,3] 盛占武 JI Yuxue;ZHENG Lili;YANG Yang;ZHONG Shuang;AI Binling;ZHENG Xiaoyan;XIAO Dao;SHENG Zhanwu(College of Horticulture,Hainan University,Haikou 570228,China;Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou 570102,China;Haikou Key Laboratory of Banana Biology,Haikou 570102,China)

机构地区：[1]海南大学园艺学院,海南海口570228 [2]中国热带农业科学院海口实验站,海南海口570102 [3]海口市香蕉生物学重点实验室,海南海口570102

出　　处：《食品科学》2022年第2期18-26,共9页Food Science

基　　金：国家自然科学基金面上项目(31772096);中央级公益性科研院所基本科研业务费专项(17CXTD-05,1630092019001);海南省自然科学基金创新团队项目(320CXTD440)。

摘　　要：采用乳化-蒸发法制备负载3种多酚的蛋白复合物纳米颗粒。先将阿魏酸(ferulic acid,FA)、槲皮素(quercetin,QT)和香草酸(vanillic acid,VA)与热处理的β-乳球蛋白(β-lactoglobulin,β-LG)结合形成三配体复合物,再利用乳化-蒸发法进一步降低复合物粒径。通过动态光散射、圆二色谱、红外色谱、荧光光谱和透射电子显微镜探究复合物的结构和形态,并利用超高效液相色谱检测复合物中多酚的加载率和稳定性。结果表明,乳化-蒸发法成功将β-LG多酚三配体复合物粒径由亚微米级(100~200 nm)降至纳米级(<100 nm);利用荧光光谱、红外色谱和圆二色谱证实了3种多酚与β-LG结合后改变了β-LG的微环境和二级结构;透射电子显微镜图像显示复合物呈椭圆形且分布均匀。复合物纳米颗粒中FA、QT、VA加载率分别为86.78%、75.00%、86.40%,高于三配体复合物的加载率60.11%、37.09%、68.93%,且纳米颗粒稳定性更强。In this study, β-lactoglobulin(β-LG) nanoparticles loaded with three polyphenols were prepared by emulsification-evaporation method. Ferulic acid, quercetin and vanillic acid were combined with heat-treated β-LG to form a three-ligand complex, and then the emulsification-evaporation method was used to reduce the particle size of the complex. The structure and morphology were investigated by dynamic light scattering, circular dichroism(CD) spectroscopy, infrared spectroscopy,fluorescence spectroscopy, and transmission electron microscopy(TEM). The polyphenol loading efficiency and stability were studied by ultra-high performance liquid chromatography(UPLC). The results showed that the particle size of the β-lactoglobulin-polyphenol complex was reduced from the submicron level(100–200 nm) to the nanometer level(< 100 nm)by the emulsification-evaporation method. Fluorescence, infrared and CD spectra showed that the addition of polyphenols changed the microenvironment and secondary structure of β-LG. TEM images showed that the composite was approximately elliptical in shape and evenly distributed. The loading efficiencies of ferulic acid, quercetin and vanillic acid in the composite nanoparticles were 86.78%, 75.00% and 86.40%, respectively, which were 60.11%, 37.09% and 68.93% higher than those in the three-ligand complex, respectively and the former had better stability.

关 键 词：纳米颗粒 Β-乳球蛋白 多酚 乳化-蒸发 粒径 

分 类 号：TS206.4[轻工技术与工程—食品科学]