Calcium silicate enhances immunosuppressive function of MSCs to indirectly modulate the polarization of macrophages  

作　　者：Haiyan Li Wenrui Wang Jiang Chang 

机构地区：[1]School of Biomedical Engineering,Shanghai Jiao Tong University,1954 Huashan Road,Shanghai 200030,China [2]Chemical and Environment Engineering Department,School of Engineering,RMIT University,124 La Trobe Street,Melbourne,VIC 3001,Australia [3]State Key Laboratory of Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,1295 Dingxi Road,Shanghai 200050,China

出　　处：《Regenerative Biomaterials》2021年第6期136-147,共12页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31771024 and 31971274).

摘　　要：Bioactive silicate ceramics(BSCs)have been widely reported to be able to induce bone tissue regeneration,but the underlying mechanisms have not been fully elucidated.Previous studies have reported that ionic products of BSCs can promote bone regeneration by directly simulating osteogenic differentiation of mesenchymal stem cells(MSCs)and modulating the polarization of macrophages to create a favorable inflammation microenvironment for initiating bone regeneration cascades.However,the immunomodulatory ability of MSCs also plays a critical role in bone regeneration but the effects of BSCs on the immunomodulatory ability of MSCs have been rarely investigated.This study aims to investigate the effects of ionic products of BSCs on the immunoregulatory ability of MSCs to further understand the mechanism of BSCs enhancing bone regeneration.Results showed that ionic products of calcium silicate(CS),one of the representative BSCs,could enhance the immunosuppressive function of human bone marrow mesenchymal stem cells(HBMSCs)by up-regulating the expression of immunosuppressive factors in HBMSCs via NF-jB pathway.In addition,CS-activated HBMSCs showed stronger stimulatory effects on M2 polarization of macrophages than CS ionic products.Furthermore,the macrophages educated by CSactivated HBMSCs showed stronger stimulatory effects on the early osteogenic differentiation of HBMSCs than the ones regulated by CS ionic products.These results not only provide further understanding on the mechanism of BSCs enhancing bone regeneration but also suggest that it is critical to consider the effects of biomaterials on the immunomodulatory function of the tissue forming cells when the immunomodulatory function of biomaterials is investigated.

关 键 词：calcium silicate MSCS MACROPHAGE IMMUNOMODULATION 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]