高效液相色谱法检测食品中生物胺的不同离线衍生策略的比较分析  

Comparative analysis of different off-line derivatization strategies for the determination of biogenic amines in food by high performance liquid chromatography

作　　者：杨静 刘宗科 邹鹏 王琨 李艳莉 郭春锋[3] YANG Jing;LIU Zong-Ke;ZOU Peng;WANG Kun;LI Yan-Li;GUO Chun-Feng(Yangling Quality and Technical Inspection Center,Yangling 712100,China;Meixian Food Safety Inspection Center,Meixian 722300,China;College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China)

机构地区：[1]杨凌质量技术检测检验所,杨凌712100 [2]眉县食品安全检验检测中心,眉县722300 [3]西北农林科技大学食品科学与工程学院,杨凌712100

出　　处：《食品安全质量检测学报》2021年第23期9036-9043,共8页Journal of Food Safety and Quality

基　　金：陕西省市场监督管理局科技计划项目(2019KY13)。

摘　　要：目的比较和分析高效液相色谱法检测食品生物胺过程中最常用的4种离线衍生策略。方法分别采用丹磺酰氯、9-芴甲基氯甲酸酯、苯甲酰氯、4-二甲胺基苯基偶氮苯磺酰氯对混合酱油样品和混合葡萄酒样品中的生物胺进行衍生,分别于衍生后的0、24和48 h用紫外检测器-反相高效液相色谱法定量分析生物胺衍生物的水平。结果4种衍生剂均能与腐胺、尸胺、精胺、亚精胺、酪胺、β-苯乙胺、组胺、色胺的胺基反应,但衍生产物的稳定性不同,采用丹磺酰氯制备的生物胺衍生物最稳定,在室温下放置48h未发现显著降解,而采用其他衍生剂制备的某些特定生物胺衍生物则发生了显著降解。此外,采用不同衍生策略制备的8种生物胺衍生物在C_(18)色谱柱上的洗脱顺序也存在差异。结论采用丹磺酰氯制备的生物胺衍生物具有高稳定性,适合在高效液相色谱仪上采用批处理程序进行定量分析,而采用其他3种衍生剂制备的某些特定生物胺的衍生物稳定性不佳,不适合在高效液相色谱仪上采用批处理程序进行定量分析。Objective To compare and analyze 4 kinds of most commonly used off-line derivatization strategies in the detection of food biogenic amines by high performance liquid chromatography.Method Biogenic amines in both mixed soy sauce samples and mixed red wine samples were derivatized with dansyl chloride,9-fluoromethoxycarnonyl chloride,benzyol chloride and dabsyl chloride,respectively.Subsequently,the levels of biogenic amine derivatives were quantitatively analyzed by ultraviolet detector reversed phase high performance liquid chromatography at 0,24 and 48 hours after derivatization.Results Four kinds of derivatives could react with putrescine,cadaverine,spermine,spermidine,tyramine,β-phenylethylamine,histamine and tryptamine,but the stability of the reaction products was different.The biogenic amine derivatives prepared with dansyl chloride were the most stable,and no significant degradation was found after 48 hours at room temperature,while some specific biogenic amine derivatives prepared with other derivatives were significantly degraded.In addition,the elution sequence of 8 kinds of biogenic amine derivatives prepared by different derivatization strategies on C_(18) column was also different.Conclusion The biogenic amine derivatives prepared by dansyl chloride have high stability and are suitable for quantitative analysis using batch program on high performance liquid chromatograph.However,the derivatives of some specific biogenic amines prepared by other 3 kinds of derivatives have poor stability and are not suitable for quantitative analysis using batch program on high performance liquid chromatograph.

关 键 词：生物胺 衍生 丹磺酰氯 9-芴甲基氯甲酸酯 苯甲酰氯 4-二甲胺基苯基偶氮苯磺酰氯 

分 类 号：O657.72[理学—分析化学] TS207.3[理学—化学]