东紫苏多酚提取物对HepG2细胞氧化损伤的保护作用研究  

Study on the protective effect of polyphenol extract from Elsholtzia bodinieri Vaniot against oxidative damage of HepG2 Cells

作　　者：刘先皓 杨明静 陈壁 黄勇桦 张建平 初雅洁 李淳 杨士花 鲁朝凤 罗天蓉 李永强[1] LIU Xian-Hao;YANG Ming-Jing;CHEN Bi;HUANG Yong-Hua;ZHANG Jian-Ping;CHU Ya-Jie;LI Chun;YANG Shi-Hua;LU Chao-Feng;LUO Tian-Rong;LI Yong-Qiang(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;College of Tea(Pu’er),West Yunnan University of Applied Sciences,Pu’er 665099,China;Yunnan Kunming State Grain Reserve Bank Transter,Kunming 650201,China;Dali Vocational and Technical College of Agriculture and Forestry,Dali 671003,China;Yongping Comprehensive Test Institute,Yongping 672660,China;Library of Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,昆明650201 [2]滇西应用技术大学普洱茶学院,普洱665099 [3]云南昆明国家粮食储备中转库,昆明650201 [4]大理农林职业技术学院,大理671003 [5]永平县综合检验检测院,永平672660 [6]云南农业大学图书馆,昆明650201

出　　处：《食品安全质量检测学报》2021年第24期9499-9506,共8页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31560428、31360378)。

摘　　要：目的研究东紫苏(Elsholtzia bodinieri Vaniot)多酚提取物对H_(2)O_(2)诱导的HepG2细胞氧化应激损伤的保护作用。方法采用福林酚(Folin-Ciocalte)法测定东紫苏提取物的多酚含量,通过测定1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除能力(DPPH radical scavenging activity, DRSA)、铁离子还原/抗氧化能力(ferric reducing antioxidant power, FRAP)、过氧化氢清除活性(hydrogen peroxide scavenging activity,HPSA)和总抗氧化能力(trolox equivalent antioxidant capacity, TEAC)来评价东紫苏多酚体外抗氧化活性,用噻唑蓝法(thiazolyl blue tetrazolium bromide,MTT)测定不同浓度的东紫苏多酚对HepG2细胞的毒性作用,并确定3个无毒性作用浓度,研究其对HepG2细胞增殖抑制作用,以800μmol/L的H_(2)O_(2)诱导HepG2细胞建立氧化应激损伤模型,通过测定HepG2细胞中活性氧(reactive oxygen species, ROS)含量、抗氧化酶[超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)]活力、谷胱甘肽(glutathione, GSH)含量以及丙二醛(malonicdialdehyde, MDA)含量的变化情况,研究在5.00、2.50和1.25μg/mL无毒性作用浓度下东紫苏多酚对HepG2细胞氧化损伤的保护作用。结果东紫苏多酚含量为(30.91±1.33)μmol/gDW,DRSA值为(17.45±0.54)μmol/gDW,FRAP值为(52.64±0.05)μmol/gDW,HPSA值为(27.12±0.17)μmol/gDW,TEAC值为(186.45±0.16)μmol/g DW。东紫苏多酚提取物能使受氧化损伤的HepG2细胞内ROS和MDA含量明显减少,并且能提高受氧化损伤细胞内GSH含量以及SOD和CAT活力。结论东紫苏多酚提取物具有较好的体外抗氧化活性,对HepG2细胞氧化应激损伤具有一定的保护作用。Objective To study the protective effect of polyphenol extract of Elsholtzia bodinieri Vaniot on oxidative stress injury of HepG2 cells induced by H_(2)O_(2).Methods The total polyphenol content was determined by Folin-Ciocalte method.1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity(DRSA),ferric reducing antioxidant power(FRAP),hydrogen peroxide scavenging activity(HPSA) and total antioxidant capacity(TEAC)were measured to evaluate the in vitro antioxidant activity of polyphenols.Thiazolyl blue tetrazolium bromide(MTT)method was used to determine the toxic effects of different concentrations of polyphenols on HepG2 cells,and 3 nontoxic concentrations were determined to study their inhibitory effects on the proliferation of HepG2 cells.HepG2 cells were induced with 800 μmol/L H_(2)O_(2)to establish an oxidative stress damage model.The content of reactive oxygen species(ROS),malondialdehyde(MDA),glutathione(GSH) and the activities of superoxide dismutase(SOD),catalase(CAT) in HepG2 cells were measured in order to study the protective effect of polyphenol extract from Elsholtzia bodinieri Vaniot oxidative damage of HepG2 cells in non-toxic concentration of 5.00,2.50 and 1.25 μg/mL.Results The total polyphenol content was(30.91±1.33) μmol/g DW,DRSA value was(17.45±0.54) μmol/g DW,FRAP value was(52.64±0.05) μmol/g DW,HPSA value was(27.12±0.17) μmol/g DW and TEAC value was(186.45±0.16) μmol/g DW respectively.The polyphenols from Elsholtzia bodinieri Vaniot reduced ROS and MDA content,and increased GSH content,SOD and CAT activity in oxidative damage HepG2 cells.Conclusion The polyphenol extract from Elsholtzia bodinieri Vaniot has good antioxidant activity in vitro,and provides a certain protective effect on oxidative stress damage of HepG2 cells.

关 键 词：东紫苏多酚 抗氧化 HEPG2细胞 氧化应激损伤 保护作用 

分 类 号：TS201.2[轻工技术与工程—食品科学]