前体物和原位萃取剂对樟芝发酵产Antrodin A和Antrodin C的影响  

Liquid Fermentation of Antrodin A and Antrodin C from Taiwanofungus camphoratus

作　　者：白旭 汪雯翰[2] 张劲松[2] 琚建伟 张守兵 丁保安 张亚平[1] 贾薇[2] BAI Xu;WANG Wenhan;ZHANG Jingsong;JU Jianwei;ZHANG Shoubing;DING Baoan;ZHANG Yaping;JIA Wei(College of Life Sciences,Shihezi Unicersity,Shihezi 832003,Xinjiang Uygur Autonomous Region,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Shanghai 201403,China;Shanghai Tongyan Industrial Co.,Ltd.,Shanghai 201417,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海201403 [3]上海彤颜实业有限公司,上海201417

出　　处：《食用菌学报》2022年第1期73-79,共7页Acta Edulis Fungi

基　　金：院企合作项目(JS0160121058)。

摘　　要：在樟芝(Taiwanofungus camphoratus)液态发酵培养基中添加不同前体物(柠檬酸、顺丁烯二酸、谷氨酰胺、苯丙氨酸、肌醇、酪氨酸)和原位萃取剂(大豆油、花生油、菜籽油、玉米胚芽油、橄榄油),测定其对樟芝发酵产Antrodin A和Antrodin C的影响。结果表明:无论是否添加前体物,发酵产物中均未检测到Antrodin A,添加0.1 g·L^(-1)的柠檬酸、顺丁烯二酸和谷氨酰胺抑制Antrodin C的产生,而添加0.1 g·L^(-1)的苯丙氨酸、肌醇、酪氨酸对Antrodin C的产量没有显著影响;添加原位萃取剂能显著促进Antrodin A的产生,而对于Antrodin C的产量,除添加玉米胚芽油的处理外,添加大豆油、花生油、菜籽油和橄榄油均使Antrodin C产量降低;同时添加原位萃取剂(大豆油)和0.1 mg·L^(-1)苯丙氨酸,Antrodin A产量达268.30 mg·L^(-1);同时添加原位萃取剂(大豆油)和0.1 mg·L^(-1)肌醇,Antrodin C产量达475.00 mg·L^(-1)。Production of Antrodin C and Antrodin A in liquid fermetation of Taiwanofungus camphoratus mycelia was studied in terms of adding different fermented precursors(citric acid,maleic acid,glutamine,phenylalanine,inositol and tyrosine)and in situ extracting agents(soybean oil,peanut oil,canola oil,corn germ oil and olive oil)to the fermentation liquid,respectively.The results showed that regardless of precursors,no Antrodin A was detected in the fermentation product.Addition of 0.1 g·L^(-1) citric acid,maleic acid or glutamine resulted in a decrease in Antrodin C yield,whereas addition of 0.1 g·L^(-1) phenylalanine,inositol or tyrosine had no significant effect on Antrodin C yield.Addition of in situ extracting agents significantly increased Antrodin A yield.On the other hand,except corn germ oil,all the other tested oils resulted in a decrease in Antrodin C yield.Antrodin A yield reached 268.30 mg·L^(-1) by simultaneous addition of soybean oil and 0.1 g·L^(-1) phenylalanine,and Antrodin C yield reached 475.00 mg·L^(-1) by simultaneous addition of soybean oil and 0.1 g·L^(-1) inositol.

关 键 词：樟芝 Antrodin A Antrodin C 原位萃取 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]