HS-SPME/GC-MS法分析大花红景天的香气成分  

Analysis of aroma components in Rhodiola crenulata by HS-SPME/GC-MS

作　　者：李涛[1] 李冲 司梦鑫 LI Tao;LI Chong;SI Mengxin(West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2022年第1期64-66,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川省科学技术厅应用基础研究计划项目(编号:2020YJ0275);四川省中医药管理局全国第四次中药资源普查科技专项资助(编号:2019PC003)。

摘　　要：目的分析川产大花红景天的挥发性香气化学成分。方法采用顶空固相微萃取结合气相色谱-质谱联用技术(HS-SPME/GC-MS)分析和鉴定大花红景天的香气化学成分,利用峰面积归一化法测定挥发性香气化学成分的相对百分含量。结果从大花红景天中分离出70种香气化学成分,鉴定出68种,占香气成分总量的97.14%,其中,含量较高的化学成分为香叶醇(25.72%)、甲酸辛酯(18.71%)、乙酸香叶酯(10.98%)、乙醋酸辛酯(5.84%)、(-)-桃金娘烯醇(5.15%)、1-癸醇(3.95%)、4-异丙基苯甲醇(3.76%)、乙酸(2.76%)、(-)-紫苏醇(2.66%)、(±)-α-乙酸松油酯(2.38%)、香茅醇(2.09%)、月桂烯(1.7%)、异戊烯醇(1.1%)。结论所用方法可为大花红景天的品质评价、质量标准制定和红景天属药用植物资源的开发利用奠定基础。OBJECTIVE To analyze the chemical constituents of volatile aroma in Rhodiola crenulata from Sichuan.METHODS Headspace solid phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME/GC-MS)was used to analyze and identify the aroma components of R.crenulata.The relative percentage content of volatile aroma components was determined by Peak area normalization method.RESULTS A total of 70 aroma chemical compounds were isolated from R.crenulata from Sichuan province,and 68 kinds of chemical components were identified,accounting for 97.14%of the total aroma components.Among them,geraniol(25.72%),octyl formate(18.71%),geranyl acetate(10.98%),octyl acetate(5.84%),(-)-myrcenol(5.15%),1-decanol(3.95%),4-isopropyl benzyl alcohol(3.76%),acetic acid(2.76%),perilla alcohol(2.66%),terpinyl acetate(2.38%),citronellol(2.09%),myrcene(1.7%),and isoamyl alcohol(1.1%).CONCLUSION This method can be used to establish the basis for quality evaluation,quality standard formulation and further development and utilization of medicinal plant resources of Rhodiola.

关 键 词：大花红景天 顶空固相微萃取 气相色谱-质谱联用 景天科 红景天属 香气成分 挥发性成分 

分 类 号：R917[医药卫生—药物分析学]