Anodal transcranial direct current stimulation alleviates cognitive impairment in an APP/PS1 model of Alzheimer’s disease in the preclinical stage  

作　　者：Yin-Pei Luo Zhi Liu Cong Wang Xiu-Fang Yang Xiao-Ying Wu Xue-Long Tian Hui-Zhong Wen 

机构地区：[1]Chongqing Key Laboratory of Neurobiology,Department of Neurobiology,School of Basic Medicine,Army Medical University,Chongqing,China [2]Chongqing Medical Electronics Engineering Technology Research Center,Laboratory of Neural Regulation and Rehabilitation Technology,College of Bioengineering,Chongqing University,Chongqing,China [3]Department of Histology and Embryology,School of Basic Medicine,Army Medical University,Chongqing,China

出　　处：《Neural Regeneration Research》2022年第10期2278-2285,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.31971287(to XYW);the Advanced Interdisciplinary Studies Foundation of School of Basic Medical Science,Army Medical University of China,No.2018JCQY07(to HZW).

摘　　要：Anodal transcranial direct current stimulation(AtDCS)has been shown to alleviate cognitive impairment in an APP/PS1 model of Alzheimer’s disease in the preclinical stage.However,this enhancement was only observed immediately after AtDCS,and the long-term effect of AtDCS remains unknown.In this study,we treated 26-week-old mouse models of Alzheimer’s disease in the preclinical stage with 10 AtDCS sessions or sham stimulation.The Morris water maze,novel object recognition task,and novel object location test were implemented to evaluate spatial learning memory and recognition memory of mice.Western blotting was used to detect the relevant protein content.Morphological changes were observed using immunohistochemistry and immunofluorescence staining.Six weeks after treatment,the mice subjected to AtDCS sessions had a shorter escape latency,a shorter path length,more platform area crossings,and spent more time in the target quadrant than sham-stimulated mice.The mice subjected to AtDCS sessions also performed better in the novel object recognition and novel object location tests than sham-stimulated mice.Furthermore,AtDCS reduced the levels of amyloid-β42 and glial fibrillary acidic protein,a marker of astrocyte activation,and increased the level of neuronal marker NeuN in hippocampal tissue.These findings suggest that AtDCS can improve the spatial learning and memory abilities and pathological state of an APP/PS1 mouse model of Alzheimer’s disease in the preclinical stage,with improvements that last for at least 6 weeks.

关 键 词：Alzheimer’s disease amyloid-β anodal transcranial direct current stimulation cognitive function INFLAMMATION long-term effect NEURON preclinical stage 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]