福建云霄地方茶树品种资源生化成分特征分析与评价  

Analysis and Evaluation on Biochemical Components of Local Tea Variety Resources in Yunxiao,Fujian

作　　者：陈潇敏 赵峰 金珊[1] 吴文晞 王鹏杰[1] 叶乃兴[1] CHEN Xiaomin;ZHAO Feng;JIN Shan;WU Wenxi;WANG Pengjie;YE Naixing(College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou 350002, China;2School of Pharmacy, Fujian University of Chinese Medicine, Fuzhou 350122, China;3Hengzheng Testing Technology Company, Fuzhou 350100, China)

机构地区：[1]福建农林大学园艺学院/茶学福建省高校重点实验室,福州350002 [2]福建中医药大学药学院,福州350122 [3]福建恒正检测技术有限公司,福州350100

出　　处：《西北植物学报》2022年第1期127-137,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项(闽教科[2015]75号);福建中医药大学引进人才科研启动项目(2801/701190097);福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A01);福建农林大学优秀硕士学位论文资助基金(1122YS01003)。

摘　　要：以福建省云霄县小帽山、梁山和南乌山3个区域共23份地方茶树品种资源为试材,采用高效液相色谱(HPLC)法和超高效液相色谱-质谱联用(UPLC-MS/MS)法测定其儿茶素、嘌呤生物碱及氨基酸组分含量,并进行描述统计分析、差异分析和聚类分析,以筛选生化成分含量特异的茶树品种资源。结果表明:(1)23份茶树品种资源的儿茶素总量变化范围为73.54~260.31 mg·g^(-1),平均值为160.48 mg·g^(-1);表没食子儿茶素-3-O-甲基-没食子酸酯(EGCG3″Me)在所有资源中均检出,含量最高的为小帽山3号(X03),高达33.65 mg·g^(-1)。(2)23份茶树品种资源的嘌呤生物碱总量变化范围为15.28~70.42 mg·g^(-1),平均值为40.95 mg·g^(-1);苦茶碱(TC)在梁山地区的茶树品种资源(LS01-LS07)均检出,其中梁山6号(L06)和梁山7号(L07)的苦茶碱含量高于其咖啡碱含量。(3)23份茶树品种资源的氨基酸总量变化范围为6.40~47.57 mg·g^(-1),平均值为20.83 mg·g^(-1);氨基酸构成丰富,共检测出20种氨基酸物质。(4)差异分析和聚类分析结果显示,云霄县3个区域茶树资源的生化成分具有明显地域特征。其中梁山区域茶树资源富含苦茶碱(TC),小帽山茶树资源的表没食子儿茶素没食子酸酯(EGCG)和表没食子儿茶素-3-O-甲基-没食子酸酯(EGCG3″Me)含量较高,南乌山茶树资源的丙氨酸(Ala)、谷氨酸(Glu)、谷氨酰胺(Gln)含量略高于其他两地。研究发现,云霄地方茶树品种资源的儿茶素、嘌呤生物碱及氨基酸含量差异大,具有丰富的遗传多样性,区域特征明显,筛选出高儿茶素、高EGCG3″Me、高苦茶碱、高茶氨酸等18份优特茶树品种资源,为云霄地方茶树品种资源的系统开发利用奠定了基础。A total of 23 local tea variety resources from Xiaomaoshan,Liangshan and Nanwushan in Yunxiao County,Fujian Province were used as test materials.The contents of catechins,purine alkaloids and amino acid components detected by high performance liquid chromatography(HPLC)and ultra performance liquid chromatography-mass spectrometry(UPLC-MS/MS),and descriptive statistics,difference analysis and cluster analysis were performed for screening tea varieties with specific biochemical components.The results showed that:(1)the total catechin content of 23 tea variety resources ranged from 73.54-260.31 mg·g^(-1),with an average of 160.48 mg·g^(-1).Epigallocatechin-3-O-methyl-gallate(EGCG3″Me)was detected in all resources,and the highest content was Xiaomaoshan 3(X03),up to 33.65 mg·g^(-1).(2)The total purine alkaloids in 23 tea variety resources ranged from 15.28-70.42 mg·g^(-1),with an average of 40.95 mg·g^(-1).The theacrine(TC)was detected in tea variety resources(LS01-LS07)in Liangshan,and the theacrine content of Liangshan 6(L06)and Liangshan 7(L07)were higher than their caffeine contents.(3)The total amino acid content of 23 tea variety resources ranged from 6.40-47.57 mg·g^(-1),with an average of 20.83 mg·g^(-1).Amino acid composition is rich,a total of 20 amino acids were detected.(4)The results of difference analysis and cluster analysis showed that the biochemical components of tea variety resources in three regions of Yunxiao County had obvious regional characteristics.Among them,tea resources in Liangshan are rich in theacrine(TC).The contents of epigallocatechin gallate(EGCG)and epigallocatechin-3-O-methyl-gallate(EGCG3″Me)were higher in Xiaomaoshan resources.The contents of alanine(Ala),glutamic acid(Glu)and glutamine(Gln)in Nanwushan tea variety resources were slightly higher than those in other two places.Our data suggested that the catechins,purine alkaloids and amino acids of Yunxiao local tea variety resources were significantly different,rich genetic diversity and obvious regional characterist

关 键 词：茶树 品种资源 苦茶碱 EGCG3″Me 茶氨酸 云霄 

分 类 号：Q946.8[生物学—植物学] S571.1[农业科学—茶叶生产加工]