信号分子AI-2合成关键基因luxS对副溶血性弧菌生物学特性的影响  

Effects of the critical gene lux S related to synthesis of AI-2 on biological characteristics of Vibrio parahaemolyticus

作　　者：江艳华[1] 王婷[1,2] 林才云 王鹏 李风铃[1] 曲梦[1] 王联珠 谭志军[1] 姚琳[1] JIANG Yanhua;WANG Ting;LIN Caiyun;WANG Peng;LI Fengling;QU Meng;WANG Lianzhu;TAN Zhijun;YAO Lin(Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,Shandong,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266003,Shandong,China)

机构地区：[1]中国水产科学研究院黄海水产研究所农业农村部水产品质量安全检测与评价重点实验室,山东青岛266071 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《微生物学通报》2022年第2期690-701,共12页Microbiology China

基　　金：中国水产科学研究院黄海水产研究所基本科研业务费(20603022020004);中国水产科学研究院基本科研业务费(2020TD71);国家重点研发计划(2017YFC1600703)。

摘　　要：[背景]副溶血性弧菌是全球范围重要的食源性病原菌,能引起急性肠胃炎。群体感应系统LuxS/AI-2影响细菌的生物学特性,为研究副溶血性弧菌的传播机制和控制技术提供了新的途径。[目的]探讨群体感应信号分子AI-2合成关键基因luxS对海产品中分离的副溶血性弧菌Vp2009027生物学特性的影响。[方法]利用自杀质粒同源重组技术敲除信号分子AI-2合成关键基因luxS,构建副溶血性弧菌Vp2009027的luxS基因缺失株,通过比较野生株与luxS基因缺失株的生长曲线、AI-2活性、运动能力、生物膜形成能力和耐药性,分析LuxS/AI-2系统对副溶血性弧菌生物学特性的影响。[结果]构建了副溶血性弧菌Vp2009027的luxS基因缺失株,野生株和luxS基因缺失株的生长无明显差异,luxS基因的缺失导致AI-2合成受阻、运动能力和生物膜形成能力增强、四环素耐药性降低。[结论]luxS基因对副溶血性弧菌的生物学特性具有重要的调控作用,为进一步研究副溶血性弧菌的传播机制和研发控制技术提供基础。[Background]Vibrio parahaemolyticus is an important foodborne pathogen worldwide,which can cause gastroenteritis.Quorum sensing system LuxS/AI-2 can regulate the biological characteristics of bacteria,which provides a new pathway to study the dissemination mechanism and control techniques of V.parahaemolytics.[Objective]To study the effect of quorum sensing signaling molecule autoinducer-2(AI-2)on the biological characteristics of V.parahaemolyticus Vp2009027 from seafood.[Methods]The critical gene luxS related to the synthesis of AI-2 was knocked out,and the luxS-knockout mutant of V.parahaemolytics Vp2009027 was constructed by homologous recombination with suicide plasmid.The effect of LuxS/AI-2 on the biological characteristics of V.parahaemolyticus was determined by comparing the growth,AI-2 activity,motility,biofilm formation ability,and antibiotic resistance between the wildtype strain and the mutant.[Results]The mutant was constructed successfully,which showed no significant difference in growth compared with the wildtype strain.The deletion of luxS led to the failure of AI-2 synthesis,increase in motility and biofilm formation ability,and weakening of tetracycline resistance.[Conclusion]luxS gene could regulate the biological characteristics of V.parahaemolyticus,which provided a reference for further research on the dissemination mechanism and development of control techniques of V.parahaemolyticus.

关 键 词：副溶血性弧菌 LUXS基因 信号分子AI-2 生物膜 四环素耐药性 

分 类 号：S941.42[农业科学—水产养殖]