戊糖乳杆菌DF9对副溶血弧菌群体感应的淬灭作用  

Quenching Effects of Lactobacillus pentosus DF9 on Quorum Sensing of Vibrio parahaemolyticus

作　　者：上官文丹 陈可 陈清莹 张杏果 钟青萍 SHANGGUAN Wendan;CHEN Ke;CHEN Qingying;ZHANG Xingguo;ZHONG Qingping(SCAU(Chaozhou)Food Institute Co.,Ltd.,Chaozhou 521000,China;Guangdong Provincial Key Laboratory of Food Quality and Safety,College of Food Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华农(潮州)食品研究院有限公司,广东潮州521000 [2]广东省食品质量与安全重点实验室,华南农业大学食品学院,广东广州510642

出　　处：《食品工业科技》2022年第6期126-132,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金面上项目(31972046);广东省自然科学基金项目(2021A1515011083);国家重点研发计划(2017YFC1601203);广东省科技计划项目(2020B1212060059)。

摘　　要：以副溶血弧菌的群体感应(quorum sensing,QS)为靶标,研究戊糖乳杆菌DF9的乙酸乙酯提取物作为群体感应抑制剂(QS inhibitor,QSI)对其的淬灭效果。在确定DF9-QSI对副溶血弧菌和哈维氏弧菌BB170生长影响的基础上,探讨亚抑菌浓度的DF9-QSI对副溶血弧菌QS信号分子AI-2的活性、动力(群集、泳动和蹭行)、生物被膜形成和胞外多糖合成的影响。结果表明,当DF9-QSI≤0.8 mg/mL时,其不影响副溶血弧菌的生长,且可有效降低其AI-2活性,抑制副溶血弧菌的运动、生物被膜形成及胞外多糖的产生,呈现浓度依赖性。三种显微结果(光学显微镜、扫描电镜和激光共聚焦显微镜)联合表明DF9-QSI对副溶血弧菌生物被膜的破坏作用。因此,本研究发现DF9-QSI具有良好的AI-2类QS淬灭活性,为开发一种新型、有效的乳酸菌源QSIs提供理论依据,也为控制副溶血弧菌QS系统提供潜在的微生物资源。The quenching effects of ethyl acetate extract of Lactobacillus pentosus DF9 as quorum sensing inhibitor on the quorum sensing(QS) of Vibrio parahaemolyticus were studied. Based on determining the effects of DF9-QSI on the growth of V. parahaemolyticus and V. harveyi BB170, the effects of DF9-QSI at sub-inhibitory concentration on QS signal molecule AI-2 activity, motility(swarming, swimming and twitching), biofilm formation and extracellular polysaccharide(EPS) synthesis of V. parahaemolyticus were investigated. The results showed that DF9-QSI had no effect on the growth of V. parahaemolyticus at the concentration lower than 0.8 mg/mL, but effectively reduced the AI-2 activity, and inhibited the movement, biofilm formation and EPS synthesis of V. parahaemolyticus in a concentration-dependent manner. The destructive effects of DF9-QSI on biofilms were further confirmed by the observations using optical microscope, scanning electron microscope and laser confocal microscope. These results indicated that DF9-QSI possessed AI-2 QS quenching activity, which might provide the theoretical basis for developing a novel QSI from lactic acid bacteria, and supply the potential microbial resources for controlling QS system of V. parahaemolyticus.

关 键 词：副溶血弧菌 戊糖乳杆菌 群体感应抑制剂 生物被膜 

分 类 号：TS201.3[轻工技术与工程—食品科学]