响应面法优化花生四烯酸油酶法脱胶工艺研究  

Optimization of enzymatic degumming of arachidonic acid oil by response surface methodology

作　　者：郭婷婷[1] 万楚筠[1] 黄凤洪[1] 魏春磊 刘锐 黄家章 GUO Ting-ting;WAN Chu-yun;HUANG Feng-hong;WEI Chun-lei;LIU Rui;HUANG Jia-zhang(Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan 430062,Hubei,China;Institute of Food and Nutrition Development,Ministry of Agriculture and Rural Affairs,Beijing 100081,China)

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业农村部食物与营养发展研究所,北京100081

出　　处：《粮食与油脂》2022年第3期85-88,共4页Cereals & Oils

基　　金：中国农业科学院科技创新工程(CAAS-ASTIP-2016-OCRI);中国农业科学院科技创新工程协同创新任务“主要农产品营养品质评价与调控”(CAAS-XTCX20190025);科技成果转化专项(2019030703011505)。

摘　　要：为确定花生四烯酸油酶法脱胶工艺的最佳条件,研究了磷脂酶A1用量、加水量及酶解时间对脱磷率的影响,在此基础上,采用Box-Behnken试验构建多元二次回归模型,并通过方差分析得到酶法脱胶工艺的最佳参数为磷脂酶A1用量510 U/kg、加水量3.3 g/100 g、酶解时间140 min,在该条件下脱磷率为96.90%。In order to determine the optimum conditions of enzymatic degumming of arachidonic acid oil,the effects of phospholipase A1 dosage,water addition and enzymatic hydrolysis time on the dephosphorization rate were studied.Based on these,multiple quadratic regression model was established by Box-Behnken test.Through analysis of variance,it was found that the optimum parameters of enzymatic degumming were phospholipase A1 dosage 510 U/kg,water amount 3.3 g/100 g and enzymatic hydrolysis time140 min.Under these conditions,the dephosphorization rate was 96.90%.

关 键 词：花生四烯酸油 酶法脱胶 响应面法 

分 类 号：TS224.6[轻工技术与工程—粮食、油脂及植物蛋白工程]