低产高级醇工业上面发酵酵母的选育  

Breeding of industrial fermentation yeast with low yield of higher alcohol

作　　者：冯鹏鹏 周钰涵 高杏 高滢 郭立芸 葛峻伶 林良才 张翠英[1,2,3,4] FENG Pengpeng;ZHOU Yuhan;GAO Xing;GAO Ying;GUO Liyun;GE Junling;LIN Liangcai;ZHANG Cuiying(State Key Laboratory Nutrition and Safety,Tianjin 300457,China;Key Laboratory of Fermentation Microbiology of Ministry of Education,Tianjin 300457,China;Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China;College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Technology Center of Beijing Yanjing Beer Co.,Ltd.,Beijing Key Laboratory of Beer Brewing Technology,Beijing 101300,China)

机构地区：[1]省部共建食品营养与安全国家重点实验室(天津科技大学),天津300457 [2]工业发酵微生物教育部重点实验室(天津科技大学),天津300457 [3]天津市工业微生物重点实验室(天津科技大学),天津300457 [4]天津科技大学生物工程学院,天津300457 [5]北京燕京啤酒股份有限公司技术中心,啤酒酿造技术北京市重点实验室,北京101300

出　　处：《食品与发酵工业》2022年第5期23-28,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31471724);天津市应用基础与前沿技术研究计划项目(14JCZDJC32900)。

摘　　要：为了获得低产高级醇的工业酿酒酵母菌株,以酿酒酵母(Saccharomyces cerevisiae)菌株680bg为出发菌株,运用常压室温等离子体(atmospheric and room temperature plasma, ARTP)对其进行不同时长的诱变处理,结合孔板培养和分光光度法检测,建立了高通量筛选体系。最终,筛选获得了8株低产高级醇的酵母菌株,其中菌株ARTP-162的高级醇产量下降最为显著,降低了约21%,且遗传性能稳定。In order to obtain the industrial Saccharomyces cerevisiae strains with lower yield of higher alcohols, artificial evolution by atmospheric room temperature plasma(ARTP) was carried out. The high-throughput screening was also developed, which combined microplate culture system and spectrophotometry analysis. After two rounds of screening, eight mutants with lower yield of high alcohol were obtained. Among them, ARTP-162 showed the lowest higher alcohol concentration, which was decreased by around 21%. Furthermore, this mutant remained genetically stable after eight generations.

关 键 词：酿酒酵母 高级醇 高通量筛选 遗传稳定性 ARTP 

分 类 号：TS261.11[轻工技术与工程—发酵工程]