牡蛎精氨酸激酶过敏原小鼠过敏模型的建立与分析  

Establishment and analysis of oyster arginine kinase allergy mice model

作　　者：赵晓涵 李国明[1] 程青丽 冯晓文 卢知浩 张新雪 谷瑞增[1] 刘文颖[1] 唐艳斌 ZHAO Xiaohan;LI Guoming;CHENG Qingli;FENG Xiaowen;LU Zhihao;ZHANG Xinxue;GU Ruizeng;LIU Wenying;TANG Yanbin(China National Research Institute of Food&Fermentation Industry,Beijing Engineering Research Center of Protein and Functional Peptides,Beijing 100015,China;National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Key Laboratory of TraceElement Nutrition,National Health and Family Planning Commission of the People’s Republic of China,Beijing 100050,China)

机构地区：[1]中国食品发酵工业研究院,北京市蛋白功能肽工程技术研究中心,北京100015 [2]中国疾病预防控制中心营养与健康所,国家卫生计生委微量元素重点实验室,北京100050

出　　处：《食品与发酵工业》2022年第5期41-46,共6页Food and Fermentation Industries

基　　金：中国食品发酵工业研究院强院工程培育专项(院强院20-功能肽-505);国家自然科学基金项目(31671963)。

摘　　要：食物引起的过敏反应发病率正在增加,这对人们的健康构成了极大的威胁。为了评价牡蛎精氨酸激酶(arginine kinase, AK)过敏原的致敏性,利用AK口服灌胃和腹腔注射2种方式使BALB/c小鼠致敏。观察所有BALB/c小鼠的过敏反应、体重及血项指标情况,利用酶联免疫吸附法检测免疫球蛋白E(immunoglobulin E,IgE)的变化,使用苏木精-伊红及甲苯胺蓝染色观察脏器病变。结果表明,实验组小鼠都产生过敏反应,对照组无明显症状,实验组小鼠体重低于对照组,血液中细胞含量增加。此外,免疫期间实验组IgE含量都比对照组高,染色切片说明实验组小鼠组织出现较严重的损伤,空肠肥大细胞脱颗粒情况严重。该实验获得了牡蛎AK致敏血清,而且成功构建牡蛎AK的BALB/c小鼠过敏模型,为贝类过敏机制和临床免疫治疗提供了有意义的研究基础。The incidence of food-induced allergic diseases is increasing, which poses a great threat to people’s health. To evaluate the sensitivity of oyster allergen arginine kinase(AK), AK was intragastric and intraperitoneal injected to sensitize BALB/c mice to established mouse model. The allergic reaction, body weight, and blood index of all BALB/c mice were explored. The changes of IgE were detected by ELISA. The variations of tissues and organs were observed by hematoxylin-eosin(HE) and toluidine blue staining. The results revealed that all mice in the experimental group had allergic reaction, while the control group shows no obvious symptoms. The body weight of the experimental group was lower than that of the control group, and the number of cells in the blood increased. Furthermore, the IgE contents of the experimental group were higher than control group during immune period. The staining results showed that the experimental group displayed severe lesion damage in the organs, and the jejunal mast cells degranulation was serious. In this assay, AK sensitized serum is obtained, and the oyster AK BALB/c mouse allergy model is successfully constructed, which provides a significant research foundation for the shellfish allergy mechanism and clinical immunotherapy.

关 键 词：牡蛎精氨酸激酶 小鼠过敏模型 IGE 组织病变 肥大细胞 

分 类 号：TS201.6[轻工技术与工程—食品科学] TQ460.1[轻工技术与工程—食品科学与工程]