基于超高效液相色谱-四极杆-飞行时间高分辨质谱分析酸橙果实中的生物活性成分  

Analysis of bioactive compounds in sour orange fruit based on UPLC-Q-TOF-HRMS

作　　者：秦艳 赵希娟 郭鹏妹[1] 庞雯辉 董超 张耀海 王成秋[1] 赵其阳[1] 焦必宁[1] QIN Yan;ZHAO Xijuan;GUO Pengmei;PANG Wenhui;DONG Chao;ZHANG Yaohai;WANG Chengqiu;ZHAO Qiyang;JIAO Bining(Laboratory of Risk Assessment for Citrus Quality and Safety,Quality Supervision and Testing Center for Citrus and Seedling,Key Laboratory of Quality and Safety Control for Citrus fruits,Ministry of Agriculture and Rural Affairs,Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China;College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China)

机构地区：[1]中国农业科学院/西南大学柑桔研究所,农业农村部柑桔产品质量安全风险评估实验室(重庆),农业农村部柑桔及苗木质量监督检验测试中心,农业农村部柑橘类果品质量安全控制重点实验室,重庆400712 [2]西南大学园艺园林学院,重庆400715

出　　处：《食品与发酵工业》2022年第5期268-274,共7页Food and Fermentation Industries

基　　金：国家重点研发专项(2019YFC1605604);国家现代农业(柑桔)产业技术体系建设专项(CARS-26);国家农产品质量安全风险评估重大专项(GJFP20210204)。

摘　　要：应用超高效液相色谱-四极杆-飞行时间高分辨质谱结合靶向和非靶向筛查方法系统分析鉴定了6个酸橙果实品种的不同部位(果皮、果肉、果汁)的生物活性物质。根据化合物的精确分子质量、质谱裂解规律、碎片离子信息,结合数据库匹配、标准品比对并参考相关文献对化合物结构进行鉴定。该实验共鉴定了84种生物活性成分,包括44种类黄酮、8种酚酸、22种香豆素、2种类柠檬苦素、8种生物碱,其中35种生物活性成分首次在酸橙中报道,进一步丰富了酸橙生物活性成分的数据库。小叶酸橙、代代酸橙、万木橙、蚌柑、摩洛哥酸橙和黄皮酸橙分别筛查鉴定出72、71、68、71、71和66种生物活性成分。通过层次聚类分析发现6个酸橙品种以及果实不同部位生物活性物质代谢模式具有一定的差异,代代酸橙和蚌柑积累模式相近,小叶酸橙、万木橙、摩洛哥酸橙和黄皮酸橙之间差异较大。不同部位鉴定的生物活性成分数量呈以下规律:果皮≥果肉≥果汁。该研究结果可为酸橙的质量评价和药效物质基础研究提供实验依据,并为其后续的资源开发奠定基础。Targeted and non-targeted screening methods combined with UPLC-QTOF-HRMS were used to systematically analyze and identify the bioactive compounds in different parts(peel, pulp, juice) of six varieties of sour orange fruit. The compounds were identified based on the accurate molecular mass, MS/MS fragment ions and MS/MS fragmentation pattern of each peak, along with database matching, standard comparison and reference. A total of 84 bioactive compounds were identified in sour orange fruits, including 44 flavonoids, 8 phenolic acids, 22 coumarins, 2 limonoids and 8 alkaloids. In which, 35 bioactive compounds were first reported, and could further enrich the database of bioactive compounds in sour orange. Furthermore, 72, 71, 68, 71, 71 and 66 bioactive compounds were identified by screening from Citrus aurantium L. cv. Xiaoye, Citrus aurantium L. cv. Daidai, Citrus aurantium L. cv. Wanmu, Citrus aurantium reuculata blaoco, Citrus aurantium L. cv. Morocco sour orange, Citrus. arrantium L. cv. Huangpi, respectively. The results of cluster analysis showed that the patterns of Daidai sour orange and Benggan sour orang were similar, however, the difference among Xiaoye sour orange, Wanmu sour orange, Morocco sour orange and Huangpi sour orange was relatively large. Additionally, the number of bioactive compounds in different parts was investigated and the results followed the order: peel≥pulp≥juice. This study could provide the experimental basis for quality evaluation and basic research of pharmacodynamic substance, and lay a foundation for the subsequent resource development of sour orange.

关 键 词：酸橙 超高效液相色谱-四极杆飞行时间高分辨质谱 生物活性成分 鉴定 裂解途径 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]