基于RNA-seq技术的茶树新梢组培褐变分析  

Analysis of Tea Shoots Tissue Culture Browning based on RNA-seq Technology

作　　者：李解 唐敏[1,2] 翟秀明 杨娟[1,2] 侯渝嘉 Li Jie;Tang Min;Zhai Xiuming;Yang Juan;Hou Yujia(Tea Research Institute of Chongqing Academy of Agricultural Sciences,Yongchuan,402160;Chongqing Engineering Technology Research Center of Tea Industry,Yongchuan,402160)

机构地区：[1]重庆市农业科学院茶叶研究所,永川402160 [2]重庆市茶叶工程技术研究中心,永川402160

出　　处：《分子植物育种》2022年第3期943-950,共8页Molecular Plant Breeding

基　　金：本研究由重庆市科技局基础与前沿研究计划项目(cstc2019jcyj-msxmX0553);重庆市农委现代山地特色高效农业茶叶产业技术体系创新团队建设项目(2020[6]号);重庆市农业发展资金项目(NKY-2020AC001)共同资助。

摘　　要：为研究褐变对于茶树组培转录水平的影响,以褐变前后的茶树组培新梢为研究材料,利用RNA-seq技术对样品进行转录组测序分析,筛选出1 753个差异表达基因,其中上调表达590个,下调表达1 163个。通过GO和KEGG数据库,对差异基因进行功能基因注释和富集分析,发现差异基因主要富集在糖代谢、应激与防御反应、植物激素信号传导、胁迫响应等代谢通路,表明褐变对于茶树新梢生长发育相关的基础代谢造成了损伤,褐化逆境抑制了相关基因的正常表达;同时,茶树新梢在响应褐变后,激活了Glyco_ydro_1、p450、AP2等与胁迫相关的转录因子高表达。本研究表明,褐变导致茶树组培大量基因被诱导或抑制表达,与胁迫响应相关的部分转录因子显著上调表达,这为解决褐变危害并获得茶树组培成功提供帮助。In order to explore the effect of browning on the transcription level of tea plant tissue culture. The new shoots of tea plant tissue culture before and after browning were used as research materials and used RNA-seq technology to analyze the transcriptome of samples.We screened 1 753 differentially expressed genes, including590 up-regulated genes and 1 163 down-regulated genes. Through GO and KEGG database, functional gene annotation and enrichment analysis of differential genes were carried out. It was found that the differential genes were significantly enriched in the glucose metabolism, stress and defense response, plant hormone signal transduction,stress response, etc.. Indicating that browning caused damage to basic metabolism related to the growth and development of tea shoots and inhibited the normal expression of related genes. At the same time, the tea shoots in response to browning activated glyco-hydro-1 p450 and AP2 were highly expressed. The study showed that the browning induced or inhibited the expression of a large number of genes in tea shoots tissue culture and some transcription factors related to stress response were significantly up-regulated. This study provides an important theoretical basis for solving the browning damage and obtaining the success of tea tissue culture.

关 键 词：茶树(Camellia sinensis(L.)O.Ktze.) 褐变 RNA-SEQ 组培 

分 类 号：S571.1[农业科学—茶叶生产加工]