漆酶催化二氢杨梅素氧化聚合  

Oxidative Polymerization of Dihydromyricetin Catalyzed by Laccase

作　　者：刘晓玲 何承云[1] 陈春刚[1] 孙俊良[1] 刘本国[1] LIU Xiaoling;HE Chengyun;CHEN Chungang;SUN Junliang;LIU Benguo(School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China)

机构地区：[1]河南科技学院食品学院,河南新乡453003

出　　处：《食品科学》2022年第6期118-124,共7页Food Science

基　　金：国家自然科学基金面上项目(31771941,32072180);河南省自然科学基金杰出青年科学基金项目(212300410005);河南省高等学校重点科研项目计划基础研究专项(20zx016);河南省重点研发与推广专项(212102110338)。

摘　　要：系统考察漆酶催化条件对二氢杨梅素衍生物的颜色、α-葡萄糖苷酶抑制及抗氧化活性的影响,并运用波谱手段对产物结构进行表征。结果表明漆酶可用于二氢杨梅素的氧化聚合,且漆酶浓度、pH值、反应时间及温度对衍生物的颜色有规律性影响。所得二氢杨梅素衍生物的α-葡萄糖苷酶抑制活性(IC_(50)6.2μg/mL)显著高于二氢杨梅素(IC_(50)85.95μg/mL),但其1,1-二苯基-2-三硝基苯肼自由基和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力逊于二氢杨梅素。波谱分析表明漆酶通过催化二氢杨梅素酚羟基氧化,而使其以醚键结合成聚合物。本研究结果可促进二氢杨梅素在食品中的应用,并推动新型的功能性食品开发。The effects of laccase catalysis conditions on the color,α-glucosidase inhibitory activity and antioxidant activity of dihydromyricetin derivatives were systematically investigated,and the structures of the derivatives were also characterized.It was found that the oxidative polymerization of dihydromyricetin was achieved by using laccase.Laccase concentration,pH,reaction time and temperature had regular effects on the color of the derivatives.Theα-glucosidase inhibitory activity of the derivatives was significantly higher than that of dihydromyricetin,with half-maximal inhibitory concentration(IC_(50))of 6.2 versus 85.95μg/mL,but the 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)radical cation scavenging capacities were inferior to those of dihydromyricetin.Spectral analysis indicated that laccase catalyzed the oxidation of the phenolic hydroxyl groups of dihydromyricetin,consequently leading to the polymerization of dihydromyricetin by ether bonding.These results can promote the application of dihydromyricetin in foods,and the development of new functional foods.

关 键 词：二氢杨梅素 Α-葡萄糖苷酶 抗氧化 漆酶 修饰 

分 类 号：TS225[轻工技术与工程—粮食、油脂及植物蛋白工程]