安梨果渣可溶性膳食纤维微波辅助酶法提取工艺优化及结构表征  

Optimization and Characterization of Microwave-assisted Enzymatic Extraction of Soluble Dietary Fiber from Anli Fruit Pomace

作　　者：任鑫 彭飞 陈丽楠 杨越冬 REN Xin;PENG Fei;CHEN Linan;YANG Yuedong(School of Chemical Engineering,Hebei Normal University of Science&Technology,Hebei Key Laboratory of Active Components and Functions in Natural Products,Qinhuangdao 066004,China)

机构地区：[1]河北省天然产物活性成分与功能重点实验室,河北科技师范学院化学工程学院,河北秦皇岛066004

出　　处：《食品工业科技》2022年第7期191-198,共8页Science and Technology of Food Industry

基　　金：河北省重点研发计划项目(20322803D);河北省研究生创新资助项目(CXZZSS2021151)。

摘　　要：为提高安梨资源的综合利用价值,本文以安梨食品加工的副产物果渣为原料,探究安梨果渣中可溶性膳食纤维的提取工艺。在单因素实验的基础上,通过响应面法优化安梨果渣可溶性膳食纤维(ALDF)的微波辅助复合酶法最佳提取工艺,并通过扫描电镜、红外光谱和高效液相色谱对制备得到的膳食纤维进行结构表征。工艺优化结果表明:在微波功率370 W、液料比为14.4:1 mL/g、酶添加量为1.6%、pH7.0时,安梨果渣可溶性膳食纤维的得率最高,达到8.07%。扫描电镜图显示安梨果渣可溶性膳食纤维呈长约5μm的纺锤型。红外光谱显示其具有多糖的特征峰,分子量在5~2.076×10;kDa之间。研究结果表明微波辅助酶法对安梨果渣SDF有较好的提取效果,具有一定工业的应用前景,因此该方法能够为安梨果渣的高值化开发利用提供理论基础。In order to improve the comprehensive utilization value of Anli resources,the extraction technology of soluble dietary fiber from Anli pomace was studied with a by-product of food processing in this work.On the basis of single factor test,response surface methodology was used to optimize the microwave-assisted composite enzymatic extraction process of soluble dietary fiber from Anli fruit pomace(ALDF).The structure of the prepared dietary fiber was characterized by scanning electron microscopy(SEM),fourier transform infrared(FT-IR)and high performance liquid chromatography(HPLC).The optimization results showed that when the microwave power was 370 W,the liquid-to-material ratio was14.4:1 mL/g,the added amount of enzyme was 1.6%,and the pH was 7.0,the yield of ALDF was the highest level of 8.07%.The SEM showed that the ALDF had a spindle shape of about 5μm in length,FT-IR showed that it had the characteristic peak of polysaccharide,and the molecular weight ranged from 5 to 2.076×10;kDa.The results showed that the microwave-assisted enzymatic method had a good extraction effect on the ALDF with a certain industrial application prospect.Therefore,this method could provide a theoretical basis for the high-value development and utilization of Anli fruit pomace.

关 键 词：安梨果渣 可溶性膳食纤维 微波复合酶法 工艺优化 结构表征 

分 类 号：TS201.3[轻工技术与工程—食品科学]