桑叶生物碱对D-半乳糖诱导小鼠肠道菌群紊乱的调节作用  

Regulatory Effect of Mulberry Leaf Alkaloid on D-Galactose-Induced Intestinal Flora Disturbance in Mice

作　　者：贺燕 黄先智[2] 韦峥 郝麒麟 丁晓雯[1] HE Yan;HUANG Xianzhi;WEI Zheng;HAO Qilin;DING Xiaowen(College of Food Science,Southwest University,Chongqing 400716,China;Science and Technology Division,Southwest University,Chongqing 400716,China)

机构地区：[1]西南大学食品科学学院,重庆400716 [2]西南大学科技处,重庆400716

出　　处：《食品科学》2022年第5期118-126,共9页Food Science

基　　金：现代农业产业技术体系建设专项(CARS-18)。

摘　　要：目的:探究桑叶生物碱对D-半乳糖诱导小鼠肠道菌群失衡的改善作用,为桑叶生物碱的开发利用提供依据。方法:采用D-半乳糖诱导建立小鼠氧化损伤模型,然后灌胃低(50 mg/kg m_(b))、中(100 mg/kg m_(b))、高(200 mg/kg m_(b))剂量的桑叶生物碱,45 d后测定小肠组织中活性氧(reactive oxygen species,ROS)、超氧化物歧化酶(superoxide dismutase,SOD)及硫氧还蛋白-1(thioredoxin-1,Trx-1)的水平,高通量测序分析盲肠内容物中菌群的α多样性、β多样性及菌群的结构变化。结果:与模型组相比,桑叶生物碱高剂量组中ROS水平降低了30.34%(P<0.05),SOD活力及Trx-1含量分别升高了63.59%、123.75%(P<0.05)。同时,桑叶生物碱能显著改善肠道菌群的α、β多样性,调节厚壁菌门/拟杆菌门的比例,减少潜在有害菌Lachnospiraceae_NK4A136_group、螺杆菌属、Mucispirillum的相对丰度,增加潜在有益菌乳杆菌属、双歧杆菌属的相对丰度,其中高剂量桑叶生物碱的作用效果最佳。结论:桑叶生物碱可以有效改善氧化应激导致的肠道菌群的多样性下降及结构紊乱,该调节作用可能与桑叶生物碱的抗氧化活性有关。Objective:The purpose of this study was to explore the effects of mulberry leaf alkaloids on the improvement of D-galactose-induced intestinal flora imbalance in mice,so as to provide a basis for the development and utilization mulberry leaf alkaloids.Methods:D-galactose was used to establish a mouse model of oxidative damage,and then the mice were administered intragastrically with mulberry leaf alkaloids at low(50 mg/kg mb),medium(100 mg/kg mb)and high(200 mg/kg mb)doses.After 45 days of administration,the levels of reactive oxygen species(ROS),superoxide dismutase(SOD)and thioredoxin-1(Trx-1)in small intestine tissue were measured,and high-throughput sequencing was used to analyze the changes ofα-diversity,β-diversity and bacterial composition in the cecum contents of mice.Results:Compared with the model group,the level of ROS in the high-dose mulberry leaf alkaloid group was reduced by 30.34%(P<0.05),and the activity of SOD and the content of Trx-1 were increased by 63.59%and 123.75%,respectively(P<0.05).Mulberry leaf alkaloids significantly improved intestinal flora diversity,adjust the ratio of Firmicutes/Bacteroides,decrease the relative abundance of potentially harmful bacteria such as Lachnospiraceae_NK4A136_group,Helicobacter and Mucispirillum,and increase the abundance of potentially beneficial bacteria like Lactobacillus and Bifidobacterium.Among the three doses of mulberry leaf alkaloids,the high dose was the most effective.In conclusion,mulberry leaf alkaloids can effectively improve the diversity reduction and structural disorders of intestinal flora caused by oxidative stress,which may be related to the antioxidant properties of mulberry leaf alkaloids.

关 键 词：桑叶生物碱 抗氧化 D-半乳糖 肠道菌群 

分 类 号：TS201.4[轻工技术与工程—食品科学]