水杨酸缺失型突变体NahG和野生型番茄果实抗冷性的比较  

A Comparative Study on Postharvest Cold Tolerance in Salicylic Acid-Deficient Mutant(NahG)and Wild-Type Tomato Fruits

作　　者：丁洋 赵瑞瑞[2] 孙存一 申琳[2] 生吉萍[3] DING Yang;ZHAO Ruirui;SUN Cunyi;SHEN Lin;SHENG Jiping(Key Laboratory of Logistics System and Technology of Beijing,School of Logistics,Beijing Wuzi University,Beijing 101149,China;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;School of Agricultural Economics and Rural Development,Renmin University of China,Beijing 100872,China)

机构地区：[1]北京物资学院物流学院,北京市物流系统与技术重点实验室,北京101149 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]中国人民大学农业与农村发展学院,北京100872

出　　处：《食品科学》2022年第5期203-209,共7页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2016YFD0400105,2018YFC1603300)。

摘　　要：本实验以番茄水杨酸(salicylic acid,SA)缺失型突变体(NahG)和野生型(wild type,WT)的采后果实为试材,于4℃下贮藏28 d后观察番茄果实冷害症状,同时测定冷藏期间果实的冷害指数,游离脯氨酸含量,磷脂酶C(phospholipase C,PLC)、磷脂酶D(phospholipase D,PLD)、脂氧合酶(lipoxygenase,LOX)、多聚半乳糖醛酸酶(polygalacturonase,PG)和纤维素酶活力,以及木质素、纤维素、总酚和儿茶素水平。结果表明:与WT果实相比,NahG果实在冷藏后冷害症状较轻,PLC、PLD、LOX、PG和纤维素酶总体活力降低,游离脯氨酸、总酚、儿茶素含量以及木质素、纤维素质量分数升高。结论:与WT番茄果实相比,低温下SA缺失型突变株NahG果实的细胞膜和细胞壁降解减轻,抗冷相关物质含量提高,其抗冷性更强。In this study,postharvest salicylic acid(SA)-deficient mutant(NahG)and wild-type(WT)tomato fruits were stored at 4℃for up to 28 days.Chilling injury symptoms were observed,and chilling injury index,the activities of phospholipase C(PLC),phospholipase D(PLD),lipoxygenase(LOX),polygalacturonase(PG)and cellulase,and the contents of proline,lignin,cellulose,total phenols and catechinic in the fruits were measured during the storage period.Compared with the WT fruit,chilling injury index and the activities of PLC,PLD,LOX,PG and cellulase in the NahG fruit were lower,whereas the contents of proline,lignin,cellulose,total phenols and catechinic were higher.These results suggest that the degradation of cell membrane and cell wall is alleviated and the contents of cold tolerance-related compounds are increased in the NahG fruit.Therefore,it had stronger cold tolerance than the WT fruit.

关 键 词：番茄果实 水杨酸 采后 抗冷性 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]