羧甲基化南瓜多糖的制备及抗氧化、降血糖活性研究  

Preparation of carboxymethylated pumpkin polysaccharide and its antioxidant and hypoglycemic activities

作　　者：李雪晖[1] 罗心雨 王莹 LI Xue-hui;LUO Xin-yu;WANG Ying(School of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang,Henan 473061,China;Institute of Eco-environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou,Guangdong 510650,China)

机构地区：[1]南阳师范学院生命科学与农业工程学院,河南南阳473061 [2]广东省生态环境技术研究所,广东广州510650

出　　处：《食品与机械》2022年第3期178-183,246,共7页Food and Machinery

基　　金：广东省科学院平台建设专项项目(编号:2019GDASYL-0102005);国家级大学生创新创业训练计划项目(编号:202110481003X);教育部产学合作协同育人项目(编号:202101199062,202101199018)。

摘　　要：目的:提高南瓜多糖体外抗氧化活性和降血糖活性。方法:以南瓜为研究对象,考察一氯乙酸浓度、反应温度和反应时间对羧甲基化南瓜多糖取代度的影响,并进行抗氧化活性和降血糖试验。结果:羧甲基化南瓜多糖的最佳制备条件为一氯乙酸浓度1.9 mol/L、反应温度73℃、反应时间3 h,该条件下的羧甲基化多糖取代度为1.247。在一定质量浓度范围内,南瓜多糖(PP)、羧甲基化南瓜多糖(CM-PP)的抗氧化能力与质量浓度呈剂量依赖性,与修饰前南瓜多糖相比,多糖的羧甲基化修饰可以提高其对α-葡萄糖苷酶的抑制活性。结论:羧甲基化南瓜多糖的优化工艺合理可行,且具有较强的抗氧化活性和降血糖活性。Objective:To study the preparation process of carboxymethylated pumpkin polysaccharide and evaluate its antioxidant and hypoglycemic activity in vitro.Methods:Monochloroacetic acid concentration,reaction temperature and reaction time were further optimized by single factor tests and response surface tests,and the antioxidant activity test and hypoglycemic test were also carried out.Results:The optimum preparation conditions of carboxymethylated pumpkin polysaccharide were as follows:the concentration of monochloroacetic acid of 1.9 mol/L,reaction temperature of 73℃,reaction time of 3 h.Under the preparation conditions,the substitution degree of carboxymethyl polysaccharide was 1.247.Studies on antioxidant activity have shown that,within a certain range of mass concentrations,there was a dose-dependent relationship between antioxidant ability and mass concentration of pumpkin polysaccharide(PP)and carboxymethylated pumpkin polysaccharide(CM-PP).Hypoglycemic test showed that carboxymethylation of pumpkin polysaccharides also increased the inhibitory activity ofα-glucosidase compared with the pumpkin polysaccharides before modification.Conclusion:The optimized technology of carboxymethylated pumpkin polysaccharide is reasonable and feasible,and has strong antioxidant activity and hypoglycemic activity.

关 键 词：南瓜 羧甲基化 取代度 多糖 抗氧化活性 降血糖活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]