美国红鱼鱼鳞制备多肽金属螯合物的工艺优化  

Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish(Sciaenops ocellatus)

作　　者：万婧倞 吴鹏 林凌 刘源森 唐旭 徐长安 WAN Jingliang;WU Peng;LIN Ling;LIU Yuansen;TANG Xu;XU Chang’an(Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China)

机构地区：[1]自然资源部第三海洋研究所,福建厦门361005

出　　处：《食品工业科技》2022年第8期165-171,共7页Science and Technology of Food Industry

基　　金：自然资源部第三海洋研究所基本科研业务费项目(海三科2017027);烟台市海洋经济创新发展示范项目(YHCX-SW-L-201703);厦门市海洋经济创新发展示范项目(16CZY009SF05)。

摘　　要：本文研究并优化了以美国红鱼(Sciaenops ocellatus)鱼鳞为原料制备多肽及合成多肽金属螯合物的工艺条件。以美国红鱼鱼鳞为原料制备多肽,采用凝胶色谱分析多肽的分子量;利用水体系法合成多肽金属螯合物并采用EDTA络合滴定法测定金属螯合率;设计并利用L_(9)(3^(4))正交试验优化了多肽金属螯合物工艺条件;利用红外光谱检测多肽和多肽金属螯合物,验证多肽与金属的结合。凝胶色谱分析获得多肽分子量在1~6 kDa左右,正交试验获得了多肽金属螯合物的制备工艺条件,即多肽锌螯合物的工艺条件为质量比2:1、pH5.5、温度55℃、时间60 min;多肽铜螯合物的工艺条件为质量比2:1、pH6.0、温度35℃、时间55 min,在该最佳螯合条件下制备的多肽锌、铜螯合物的螯合率分别为78.21%、91.24%。红外吸收光谱结果显示Zn^(2+)、Cu^(2+)与-NH_(2)结合成功,表明多肽锌、铜螯合物是新的物质。研究结果表明,该工艺条件下的螯合率明显提高,且富含金属离子、成本低廉、市场前景可观,有利于海洋水产加工副产物高值化应用,促进渔业加工产业的技术升级。In this paper,the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish(Sciaenops ocellatus)scales were studied and optimized.Polypeptides were prepared from the scales of American red fish.The molecular weight of the peptides was analyzed by gel chromatography.Polypeptide metal chelates were synthesized by water system method and the metal chelating rate was determined by EDTA complexometric titration method.L_(9)(3^(4))orthogonal test was used to optimize the processing conditions of polypeptide metal chelates.The binding of polypeptide to metal was verified by detecting polypeptide and metal chelate by infrared spectroscopy.The molecular weight of polypeptide was about 1~6 kDa by gel chromatography analysis.The preparation conditions of polypeptide zinc chelate were obtained by orthogonal experiment,that is,the mass ratio of zinc chelate was 2:1,pH5.5,temperature 55℃,time 60 min.The optimal chelating conditions were mass ratio 2:1,pH6.0,temperature 35℃ and time 55 min.Under the optimized condition,the chelate ratio of zinc and copper were 78.21% and 91.24% respectively.The infrared absorption spectra showed that Zn^(2+),Cu^(2+) and-NH_(2) were successfully combined.The results showed that the chelation rate was significantly improved under the condition of this process,and it was rich in metal ions,low cost and promising market prospect,which would be conducive to the high-value application of marine fishery processing by-products and promote the technological upgrading of fishery processing industry.

关 键 词：鱼鳞美国红鱼 多肽 多肽金属螯合物 工艺优化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]