含DHA藻油的茶油对初断乳小鼠脑部和视网膜发育的影响  

Effect of Camellia Oil Containing DHA Algae Oil on the Development of Brain and Retina in Weaned Mice

作　　者：袁传勋 王成成 吴月 单琴 金日生 Yuan Chuanxun;Wang Chengcheng;Wu Yue;Shan Qin;Jin Risheng(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601)

机构地区：[1]合肥工业大学食品与生物工程学院,合肥230601

出　　处：《中国食品学报》2022年第3期80-87,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：安徽省科技重大专项(201903a06020024);安徽省六安市产学研合作重大专项(JZ2020 AHDS0258)。

摘　　要：以初断乳昆明小鼠为试验对象,向其灌胃含不同剂量二十二碳六烯酸(DHA)藻油的茶油,研究对小鼠脑部及视网膜发育的影响。首先通过Morris水迷宫实验进行行为学测试,探究小鼠空间和学习记忆能力;其次通过组织染色观察小鼠脑组织、海马区和视网膜结构的变化;最后测定脑组织中相关生化指标。结果表明:Morris水迷宫实验表明,与空白组相比,阳性组和DHA各剂量组小鼠的逃避潜伏期均呈现下降趋势(P<0.01)。组织染色结果显示:DHA剂量组小鼠脑组织中的细胞结构完整、细胞核及细胞质分布较为均匀,海马组织细胞数量增多,尼氏体丰富,视网膜血管分布均匀,无扭曲和聚集现象,走向规则,血管密度适中。DHA剂量组小鼠脑组织中丙二醛(MDA)含量有所下降(P<0.01),而总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活性有所增加(P<0.05,P<0.01),乙酰胆碱酯酶(A-ChE)活性呈下降趋势(P<0.05,P<0.01)。结论:含DHA藻油的茶油能提高小鼠的空间和学习记忆能力,促进小鼠脑部发育,有利于视网膜完整形态的形成。The effects of camellia oil containing different doses of docosahexaenoic acid(DHA)algal oil on the development of brain and retina of Kunming mice were studied.Firstly,Morris water maze experiment was used to conduct behavioral tests to explore the spatial and learning memory abilities of mice.Secondly,the changes of brain tissue,hippocampus and retina structure were observed by tissue staining.Finally,the related biochemical indexes in brain tissue were measured.Results:According to the Morris water maze experiment,compared with the blank group,the escape latency of mice in the positive group and each dose group of DHA showed a downward trend(P<0.01);the results of tissue staining showed that the brain tissue of mice in DHA dose group had complete cell structure,purple nucleus and relatively uniform cytoplasm distribution.The number of hippocampal tissue cells increased,Nissl body is abundant,retinal blood vessels are evenly distributed,there is no distortion or aggregation,the trend is regular,and the blood vessel density is moderate;The content of malondialdehyde(MDA)in the brain tissue of mice in the DHA dose group decreased(P<0.01),while the total antioxidant capacity(T-AOC)and catalase(CAT)activity increased(P<0.05,P<0.01),acetylcholinesterase(A-ChE)activity showed a downward trend(P<0.05,P<0.01).Camellia oil containing DHA algae oil can improve the space and learning memory abilities of mice,promote brain development in mice,and facilitate the formation of a complete retina.

关 键 词：DHA藻油 茶油 初断乳小鼠 脑发育 视网膜 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]