基于β-半乳糖苷酶的低聚半乳糖制备研究进展  

Advances in the Production of Galacto-oligosaccharides Based onβ-Galactosidase

作　　者：吴昊[1] 齐崴[1,2,3] 尤生萍[1,3] 苏荣欣 何志敏[1,2] Wu Hao;Qi Wei;You Shengping;Su Rongxin;He Zhimin(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072;State Key Laboratory of Chemical Engineering,Tianjin University,Tianjin 300072;Tianjin Key Laboratory of Membrane Science and Desalination Technology,Tianjin University,Tianjin 300072)

机构地区：[1]天津大学化工学院,天津300072 [2]化学工程联合国家重点实验室(天津大学),天津300072 [3]天津市膜科学与海水淡化技术重点实验室,天津300072

出　　处：《中国食品学报》2022年第3期329-341,共13页Journal of Chinese Institute Of Food Science and Technology

基　　金：中国博士后科学基金项目(2019M651040)。

摘　　要：低聚半乳糖(GOS)是一种广泛存在于人类母乳中的天然功能性食品成分,通过β-半乳糖苷酶(β-gal)酶法生产是目前商业上最重要的GOS来源。β-gal是一类重要的糖苷水解酶,具有水解和转糖苷功能。由于β-gal来源和应用方式的复杂性,对应的GOS产物在结构和纯度上有很大差异,因此,以高产高纯度GOS为导向的β-gal研究一直受到广泛关注。本综述从机理分析,酶种来源,催化方式角度出发,比较不同来源和应用方式的β-gal在催化GOS合成过程的表现,在此基础上展望β-gal在GOS生产中的应用发展方向。Galacto-oligosaccharides(GOS)is a natural functional food ingredient widely present in human breast milk.The production of GOS byβ-Galactosidase is main commercial source.β-Galactosidase is a kind of important glycoside hydrolase,which has the ability to simultaneously catalyze the hydrolysis reaction and the transglycoside reaction.Due to the complexity of the source and application ofβ-Galactosidase,the corresponding GOS products are very different in structure and purity.Therefore,the research ofβ-galactosidase oriented to high-yield and high-purity GOS has been receiving extensive attention.This review compares the performance ofβ-Galactosidase in the process of catalyzing GOS from the perspectives of mechanism analysis,enzyme sources,and catalytic methods.On this basis,the research direction ofβ-Galactosidase in the production of GOS is prospected.

关 键 词：低聚半乳糖 Β-半乳糖苷酶 转糖苷反应 固定化 透性化 

分 类 号：TS201.2[轻工技术与工程—食品科学]