β-葡聚糖生物强化大米28天经口毒性研究  

Twenty-eight-day oral toxicity study ofβ-glucan biofortified rice

作　　者：李沙 田丽 杨华[1] 高俊[2] 陈晓彤 王红平 阴文娅[1] LI Sha;TIAN Li;YANG Hua;GAO Jun;CHEN Xiaotong;WANG Hongping;YIN Wenya(West China School of Public Health,Sichuan University/West China Fourth Hospital,Sichuan University,Sichuan Chengdu 610041,China;Sichuan Center for Disease Control and Prevention,Sichuan Chengdu 610041,China;Sichuan Institute of Food and Drug Inspection and Testing,Sichuan Chengdu 610097,China)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,四川成都610041 [2]四川省疾病预防控制中心,四川成都610041 [3]四川省食品药品检验检测研究院,四川成都610097

出　　处：《中国食品卫生杂志》2022年第1期17-22,共6页Chinese Journal of Food Hygiene

摘　　要：目的通过28 d经口毒性试验研究,对β-葡聚糖生物强化大米的安全性进行评价。方法将断乳SD大鼠随机分为对照组和低、中、高剂量组(2.5、5.0和10.0 g/kg BW),每组22只,雌雄各半。按照10 mL/kg BW经口灌胃,连续给予28 d,实验前后对大鼠进行眼部检查,处死动物后进行尿常规、血液学、血生化、脏器系数测定及组织病理学检查。结果与对照组相比,给药组动物外观、体质量、食物利用率、脏器系数、血生化和尿常规检测均没有明显差异;雄性中、高剂量组和雌性高剂量组在第4周的进食量与对照组相比具有统计学差异(P<0.05或P<0.01),但均无时间上的延续性,认为无毒理学意义;雄性中、高剂量组的血小板含量与对照组相比具有统计学差异(P<0.05),但无剂量-反应关系,仅限单个指标异常,且均在实验室参考值范围之内,认为不具有毒理学意义;高剂量组病理学检查未见与受试物有关的病理性改变。结论在本试验条件下,SD大鼠短期经口摄入β-葡聚糖生物强化大米未见毒性作用,但长期食用的安全性尚需进一步研究。Objective To evaluate the safety ofβ-glucan biofortified rice through a 28-day oral toxicity test.Methods Weaned SD rats were randomly divided into a control group and low,medium and high dose groups(2.5,5.0 and 10.0 g/kg BW),22 in each group,half male and half female.After oral gavage(10 mL/kg BW)and continuous administration for 28 d,eyes were examined before and after the experiment.After the animals were sacrificed,urine routine,hematology,blood biochemistry,organ coefficient measurement and histopathology examination were performed.Results Compared with the control group,there were no significant differences in the appearance,weight,food utilization,organ coefficient,blood biochemistry,and urine routine tests in the sample group.Compared with the control group,the food intake of the male medium,high-dose group and the female high-dose group was statistically different(P<0.05 or P<0.01),but there was no continuity in time,which is considered to be non-toxic significance.The platelets(PLT)of the male medium and high dose groups are statistically different from the control group(P<0.05),but there is no dose-response relationship,only a single index is abnormal,and both are within the laboratory reference value range.Within,it is considered to have no toxicological significance;the pathological examination of the high-dose group showed no pathological changes related to the test substance.Conclusion Under the conditions of this experiment,short-term oral intake ofβ-glucan bio-fortified rice showed no toxic effects,but the safety of long-term consumption needs further research.

关 键 词：Β-葡聚糖 营养强化 28 d经口毒性试验 

分 类 号：R155[医药卫生—营养与食品卫生学]