植物乳杆菌XL-02发酵产γ-氨基丁酸条件的优化  

Optimization of Fermentation Conditions for γ-Aminobutyric Acid Production by Lactobacillus Plantarum XL-02

作　　者：陈显玲 莫小群 杨琴 曾婷 苏龙 Chen Xianling;Mo Xiaoqun;Yang Qin;Zeng Ting;Su Long(College of Food and Biochemical Engineering,Guangxi Science and Technology Normal University,Laibin 546199,China;Guangxi Sugar Resources Research Center of Engineering Technology,Laibin 546199,China)

机构地区：[1]广西科技师范学院食品与生化工程学院,广西来宾546119 [2]广西糖资源工程技术研究中心,广西来宾546119

出　　处：《山东化工》2022年第6期10-14,共5页Shandong Chemical Industry

基　　金：广西科技师范学院重点科研项目(GXKS2020ZD007);广西大学生创新创业训练项目(202111546008);广西大学生创新创业训练项目(202011546079)。

摘　　要：为提高γ-氨基丁酸(GABA)产量,对植物乳杆菌XL-02进行发酵条件的优化。首先,采用单因素方法对底物添加量、发酵温度、发酵时间、接种量和pH值等因素进行考察,在此基础上进行4因素3水平的响应面实验优化。结果表明:在L谷氨酸钠浓度为2.0 g/L,发酵温度30℃,接种量11%和pH值6.0的条件下,发酵60 h,γ-氨基丁酸含量由原来的0.23 g/L提高到了1.14 g/L,比优化前提高了4.96倍.此条件下理论预测值1.09 g/L,验证实验测得为1.14 g/L,与预测值接近,说明预测模型可靠性较高,可应用于γ-氨基丁酸发酵条件的优化。To improve the ability of gamma-aminobutyric acid(GABA)by Lactobacillus plantarum XL-02,L.plantarum XL-02 was optimized in this study.Firstly,using single-factor test,the effects of these 5 parameters,i.e.,the substrate addition,fermentation temperature,fermentation time,inoculation amount and intial pH of medium were studied.Then the key parameters of fermentation conditions were statistically optimized by response surface methodology with 4 factors in 3 levels.The optimal conditions were sodium hydrogen glutamate addition 2.0g/L,inoculum 11%,pH 6.0 and temperature fermentation at 30℃for 60 h.Under the conditons,the gamma-aminobutyric acid yield was 4.96 times from 0.23 g/L to 1.14 g/L.The verification test value of the gamma-aminobutyric acid 1.14 g/L,which was nearly in agreed with the theoretical prediction value 1.09 g/L.The results suggested the predicted model was reliable and available for the optimization of gamma-aminobutyric acid fermetation coditions.

关 键 词：Γ-氨基丁酸 植物乳杆菌 发酵条件 响应面 

分 类 号：TS201.3[轻工技术与工程—食品科学]