Interleukin-4 promotes microglial polarization toward a neuroprotective phenotype after retinal ischemia/reperfusion injury  

作　　者：Di Chen Cheng Peng Xu-Ming Ding Yue Wu Chang-Juan Zeng Li Xu Wen-Yi Guo 

机构地区：[1]Department of Ophthalmology,Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China [2]Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology,Shanghai,China

出　　处：《Neural Regeneration Research》2022年第12期2755-2760,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China, No.81970796(to WYG);Clinical Research Program of the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, No.JYLJ201905(to WYG);Interdisciplinary Program of Shanghai Jiao Tong University, No.YG2019QNA18(to YW)

摘　　要：Glaucoma results from irreversible loss of retinal ganglion cells(RGCs)through an unclear mechanism.Microglial polarization and neuroinflammation play an important role in retinal degeneration.Our study aimed to explore the function of microglial polarization during glaucoma progression and identify a strategy to alleviate retinal neuroinflammation.Retinal ischemia/reperfusion injury was induced in C57BL/6 mice.In a separate cohort of animals,interleukin(IL)-4(50 ng/mL,2μL per injection)or vehicle was intravitreally injected after retinal ischemia/reperfusion injury.RGC loss was assessed by counting cells that were positive for the RGC marker RNA binding protein,mRNA processing factor in retinal flat mounts.The expression of classically activated(M1)and alternatively activated(M2)microglial markers were assessed by quantitative reverse transcription-polymerase chain reaction,immunofluorescence,and western blotting.The results showed that progressive RGC loss was accompanied by a continuous decrease in M2 microglia during the late phase of the 28-day period after retinal ischemia/reperfusion injury.IL-4 was undetectable in the retina at all time points,and intravitreal IL-4 administration markedly improved M2 microglial marker expression and ameliorated RGC loss in the late phase post-retinal ischemia/reperfusion injury.In summary,we observed that IL-4 treatment maintained a high number of M2 microglia after RIR and promoted RGC survival.

关 键 词：glaucoma hyper-intraocular pressure in vivo INTERLEUKIN-4 intravitreal injection M2 microglia NEURODEGENERATION neuroprotective effect retinal ganglion cell retinal ischemia-reperfusion 

分 类 号：R775[医药卫生—眼科]