Plasma Lipidomic Analysis to Identify Novel Biomarkers for Hepatocellular Carcinoma  

作　　者：Li Yang Yu Bai Xiaohong Han Yuankai Shi Huwei Liu 

机构地区：[1]Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Beijing National Laboratory for Molecular Sciences,Institute of Analytical Chemistry,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China [2]Department of Pharmacy,Shanxi Medical University,Taiyuan 030001,China [3]Department of Medical Oncology,Cancer Institute and Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China [4]Beijing Key Laboratory of Clinical Study on Anticancer Molecular Targeted Drugs,Beijing 100021,China

出　　处：《Journal of Analysis and Testing》2017年第3期223-232,共10页分析检测（英文）

基　　金：the National Natural Science Foundation of China(Grant numbers,21527809 and 21175005).

摘　　要：Hepatocellular carcinoma(HCC)accounts for the major primary liver cancer,and its morbidity and mortality rates are very high.Identification of novel biomarkers for the early diagnosis and treatment of this disease is of crucial importance.In this study,a continuous-flow normal-phase/reversed-phase two-dimensional liquid chromatography—quadrupole time-of-flight mass spectrometry(NP/RP 2D LC-QToF/MS)method was used to detect lipid species in human plasma from HCC patients and healthy controls.As a result,the two groups were clearly separated by principal component analysis.Besides,23 lipid species with corrected p value<0.05 and fold change>1.5 were proposed as potential lipid biomarkers for HCC.Among them,9 lipid species,including galactosylceramide(36:5),free fatty acid(20:4),phosphatidylethanolamine(40:6),phosphatidylethanolamine(38:6),diacylglycerol(40:5),diacylglycerol(44:2),lactosylceramide(40:3),phosphatidylcholine(40:6),and free fatty acid(22:5),exhibited excellent ability to distinguish HCC patients from healthy controls with the area under the receiver-operating characteristic curve>0.900,showing promising clinical value.Taken together,these results indicate the great potential of NP/RP 2D LC-QToF/MS method in disease diagnosis,which might be helpful in clinical practice.

关 键 词：BIOMARKER Hepatocellular carcinoma LIPIDOMICS NP/RP 2D LC-QToF/MS 

分 类 号：R73[医药卫生—肿瘤]