寒葱不同极性溶剂萃取物的活性成分分析及抗氧化活性研究  

Analysis of active compounds and antioxidant activity of fractions from different polar solvents of Allium victorialis

作　　者：王杨欣 陈萱 范鑫博 张先[1] 李范洙[1] 李敏 WANG Yangxin;CHEN Xuan;FAN Xinbo;ZHANG Xian;LI Fanzhu;LI Min(Agricultural College of Yanbian University;Charateristic Industry Development Center of Yanbian:Yanji Jilin 133000,China)

机构地区：[1]延边大学农学院 [2]延边特色产业发展中心,吉林延吉133000

出　　处：《延边大学农学学报》2022年第1期1-8,共8页Agricultural Science Journal of Yanbian University

基　　金：吉林省重点科技攻关项目(20160204029NY);延边州科技发展攻关研究(2020FG30);延边大学大学生创新创业训练资助项目(S202010184024)。

摘　　要：为进一步分离出寒葱粗提物中的抗氧化效果较好组分,该试验以寒葱叶为原料通过水回流提取法得到粗提物,并利用正己烷、二氯甲烷、乙酸乙酯和正丁醇依次萃取,对不同极性萃取物的总酚、总黄酮及其单体物质和总皂苷质量分数进行测定,并对寒葱不同极性萃取物的抗氧化活性进行了探讨。结果表明:乙酸乙酯相的总酚、总黄酮的质量分数最高,分别为334.20和127.27 mg/g,二氯甲烷相的总皂苷质量分数最高,为139.04 mg/g。乙酸乙酯相中检测出落新妇苷、芦丁、槲皮苷、染料木素、槲皮素、山奈酚和香叶木素7种物质,且其总质量分数最高;二氯甲烷相也分析出7种物质,其总质量分数仅次于乙酸乙酯相。寒葱不同极性萃取物对DPPH自由基、羟自由基清除率和还原能力的顺序均为乙酸乙酯相>二氯甲烷相>正丁醇相>正己烷相>水相萃取物,抗氧化活性与总酚和总黄酮的质量分数呈极显著(P<0.01)正相关。本研究为天然抗氧化剂及寒葱抗氧化食品的开发提供基依据。To separate fractions of antioxidant components from the crude extracts of Allium victorialis,the leaves of A.victorialis were used to obtain the crude extract using the water reflux extract method in this experiment and the crude extract was sequentially extracted by hexane,dichloromethane,ethyl acetate,and butyl alcohol,then the contents of total phenols,total flavonoids and their several monomers,and total saponins in fractions from different polar solvents were determined.Subsequently,the antioxidant activities of the different fractions were investigated.The results showed that the highest contents of total phenols(334.20 mg/g)and total flavonoids(127.27 mg/g)were in the fraction of ethyl acetate,in which seven flavonoid monomers,including astilbin,rutin,quercetrin,genistein,quercetin,kaempferol,and diosmetin,were detected,and their contents were higher compared with other fractions.The highest content of total saponins(139.04 mg/g)was determined in the fraction of dichloromethane,and the contents of seven flavonoid monomers were lower than those of the fraction of ethyl acetate but higher than the other fractions.The order from high to low of DPPH and hydroxyl radical scavenging rates and reducing ability were ethyl acetate fraction,dichloromethane fraction,butyl alcohol fraction,hexane fraction,and water fraction.The contents of total phenols and flavonoids were significantly(P<0.01)positively correlated with the antioxidant activities.This study provided the basis for the development of natural antioxidants and antioxidant food of A.victorialis.

关 键 词：寒葱 不同极性萃取物 黄酮类物质 抗氧化 

分 类 号：TS201.21[轻工技术与工程—食品科学]