甘蔗渣酶解制糖工艺的建立与优化  

Development of an enzymatic saccharification process for sugarcane bagasse

作　　者：侯庆宇 杨前进 金瑞 张濛 田康明 王正祥 HOU Qingyu;YANG Qianjin;JIN Rui;ZHANG Meng;TIAN Kangming;WANG Zhengxiang(College of Chemical Engineering and Materials Science,Tianjin University of Science and Technology,Tianjin 300457,China;College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学化工与材料学院,天津300457 [2]天津科技大学生物工程学院,天津300457

出　　处：《食品与发酵工业》2022年第8期9-14,共6页Food and Fermentation Industries

基　　金：国家重点研发计划政府间国际科技创新合作重点专项(2018YFE0100400);天津市高等学校创新团队建设规划(TD13-5009);天津市科技计划项目(19YFZCSN00560)。

摘　　要：甘蔗渣是甘蔗工业的主要固体副产物,实现其高效生物转化的核心步骤是高效酶法水解。该研究通过酶制剂筛选与组合分析,发现β-葡萄糖苷酶(BgA)和外切葡聚糖酶(CbhC)与商品酶具有最优协同作用。将BgA和CbhC与商品酶复合,对甘蔗渣进行酶解并对复合酶酶解工艺进行研究和优化,在添加1000 U/g商品酶的基础上,添加12000 U/g BgA和0.175 U/g CbhC,50℃和pH 5.0下水解60 h的最优条件下,甘蔗渣中纤维素和半纤维素的水解率分别达到90.16%和95.65%。以此制得的甘蔗渣酶解液为碳源,进行乙醇发酵实验,乙醇产率达到30.30 g/L,转化率达到42.53%,木糖回收率为99.52%。该研究建立的酶法制糖工艺,可满足以甘蔗渣为原料生产生物乙醇的要求并可同步回收木糖。Bagasse is the main solid by-product in sugarcane industry.Enzymatic saccharification of sugarcane bagasse is a core step for its biotransformation.Through screening of enzymes and combination analysis,minimal enzyme cocktails were developed for the saccharification of sugarcane bagasse.Aβ-glucosidase(BgA)and an exo-glucanase(CbhC)demonstrated the best synergistic effects with commercial enzyme cocktails.The highest hydrolysis rates of cellulose and hemicellulose reached 90.16%and 95.65%,respectively,under the optimal conditions of hydrolysis at 50℃and pH 5.0 for 60 h,with 1000 U/g commercial enzymes,12000 U/g BgA,and 0.175 U/g CbhC.The resultant enzymatic hydrolysates were used as the carbon source in ethanol fermentation by recombinant Escherichia coli.The yield of ethanol reached 30.30 g/L,and the conversion rate of glucose and recovery rate of xylose were 42.53%and 99.52%,respectively.The preliminary results obtained in this work are promising for ethanol production with a simultaneous xylose recovery.

关 键 词：甘蔗渣 Β-葡萄糖苷酶 外切葡聚糖酶 酶解工艺 

分 类 号：TS249[轻工技术与工程—制糖工程] TQ223.122[轻工技术与工程—食品科学与工程] TQ920.6[化学工程—有机化工]