食品金属罐中14种双酚类物质迁移量的同时测定及杀菌过程对其迁移的影响  

Simultaneous Determination of Migration Amounts of 14 Kinds of Bisphenol Substances in Food Metal Cans and Effect of Sterilization on the Migration

作　　者：张宇凌 吴刚 高红波 邱逊 杨晓明 赵倩倩 徐彤彤 王亚 ZHANG Yuling;WU Gang;GAO Hongbo;QIU Xun;YANG Xiaoming;ZHAO Qianqian;XU Tongtong;WANG Ya(China National Institute of Food and Fermentation Industries Co.,Ltd.,Beijing 100015,China;Yiwu Easy Open End Industrial Co.,Ltd.,Yiwu 322015,China;National Food Management Center of China Light Industry,Beijing 100037,China;College of Food Science and Technology,Tianjin University of Science and Technology,Tianjin 300457,China;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]中国食品发酵工业研究院有限公司,北京100015 [2]义乌市易开盖实业公司,浙江义乌322015 [3]中轻食品工业管理中心,北京100037 [4]天津科技大学食品科学与工程学院,天津300457 [5]北京林业大学生物科学与技术学院,北京100083

出　　处：《食品工业科技》2022年第10期288-297,共10页Science and Technology of Food Industry

基　　金：国家重点研发计划(2018YFC1603203,2019YFC1605202/2019YFC1605200)。

摘　　要：为了调查国内食品金属罐中双酚类物质的迁移水平,本文建立了同时测定食品金属罐中14种双酚类物质迁移量的高效液相色谱法,并研究了杀菌过程对双酚类物质迁移的影响及食品金属罐中双酚类物质的迁移规律。结果表明,14种双酚类物质在4种食品模拟物(4%体积乙酸、10%体积乙醇、50%体积乙醇和异辛烷)中线性关系良好,加标回收率为83.67%~107.05%,精密度范围为2.32%~7.67%,该方法的精密度和准确度较好,可用于食品金属罐中14种双酚类物质迁移量的检测分析。对市场上9种罐头和饮料用金属罐中的14种双酚类物质的迁移量进行了同时测定,发现有7种双酚类物质检出,无双酚A及其类似物的检出。基于欧盟限量标准要求,发现午餐肉罐头中的双酚A-(2-3-二羟基丙基)缩水甘油醚(BADGE·H_(2)O)、牡蛎葛根饮料中的双酚A-二(2-3-二羟基丙基)醚(BADGE·2H_(2)O)和黑莓罐中的双酚F-(2-3-二羟基丙基醚)(BFDGE·2H_(2)O)存在一定的迁移风险。此外,杀菌过程影响食品金属罐中双酚类物质的迁移种类和迁移量。迁移试验结束后,经过杀菌的金属罐中仅检测出BADGE·2H_(2)O,而未经杀菌的金属罐中同时检测到BADGE·2H_(2)O和双酚A-(3-氯-2羟丙基)(2-3-二羟基丙基)缩水甘油醚(BADGE·H_(2)O·HCl)。另外,食品模拟物的类型影响着双酚类物质的迁移量和迁移种类,且随着迁移温度的升高和迁移时间的增加,双酚类物质的迁移量逐渐升高直至平衡。In order to investigate the migration amounts of bisphenol substances(BPs) in domestic food metal cans, a method of simultaneous detection of 14 kinds of BPs in food metal cans with high sensitivity was established by high performance liquid chromatography(HPLC). The effect of sterilization process on the migration of BPs and the migration laws of BPs in food metal cans were studied. Results showed that, all the 14 kinds of BPs were well separated and exhibited excellent linear characteristics in four kinds of food simulants(4 vol% acetic acid, 10 vol% ethanol, 50 vol% ethanol and isooctane). The precision and accuracy of the method was excellent with the recovery of the 14 kinds of BPs from83.67%~107.05% and the relative standard deviations(RSDs) from 2.32%~7.67%, indicating that HPLC method was effective for simultaneous determination of 14 kinds of BPs in food metal cans. The amounts of 14 kinds of BPs migrated from nine kinds of canned foods and beverages on the Chinese market were simultaneously determined, and found seven kinds of BPs were detected while no BPA and its analogues were detected in all samples. BADGE·H_(2)O migrating from the luncheon meat can, BADGE·2H_(2)O migrating from the oyster pueraria beverage can and BFDGE·2H_(2)O migrating from the blackberry all had the risk according to EU regulation. In addition, the sterilization process affected the amounts and types of BPs migrating from the food metal cans. After migration tests, only BADGE·2H_(2)O was detected in sterilized cans while both BADGE·2H_(2)O and BADGE·H_(2)O·HCl were detected in unsterilized cans. Moreover, the type of food simulants also affected the amount and types of BPs migrating from food metal cans. With the increasing of migration temperature or time,the migration amounts of BPs increased at first and finally kept steady when the migration reached equilibrium.

关 键 词：双酚类物质 迁移规律 杀菌 食品模拟物 高效液相色谱法(HPLC) 

分 类 号：TS297[轻工技术与工程—农产品加工及贮藏工程]