AB027.Varying pattern of proteases secretion in Fuchs corneal endothelial dystrophy  

作　　者：Isabelle Xu Mathieu Thériault Stéphanie Proulx 

机构地区：[1]Centre de Recherche en Organogénèse Expérimentale de l’UniversitéLaval/LOEX,Québec,QC,Canada [2]Centre de Recherche du CHU de Québec-UniversitéLaval,Axe Médecine Régénératrice,Québec,QC,Canada [3]Département d’Ophtalmologie et d’ORL-Chirurgie Cervico-Faciale,Facultéde Médecine,UniversitéLaval,Québec,QC,Canada

出　　处：《Annals of Eye Science》2019年第1期202-202,共1页眼科学年鉴（英文）

摘　　要：Background:The goal of this project was to analyze the relationship between cell morphology and proteases/proteases inhibitors(PIs)secretion profile in fuchs endothelial corneal dystrophy(FECD)corneal endothelial cells(CECs).Methods:Cell morphology was determined using a circularity index(4π×area/perimeter2)for each CECs population extracted from surgical FECD specimens(N=2)and healthy Eye bank corneas(N=3).CECs were cultured 28 days post-confluency.Supernatant was collected and analysed using Proteome Profiler Array detecting 35 proteases and 32 PIs(R&D Systems).Proteome signal was analyzed using Image Studio Lite and correlated with the population’s circularity index.Results:Calculation of circularity index reported different morphologies among FECD populations(0.59±0.18 and 0.64±0.17)and healthy populations(0.44±0.18,0.66±0.13 and 0.71±0.11).Proteome arrays revealed the presence of 10 proteases(ADAMTS1,Cathepsin A,B,D,and X/Z/P,DPPIV/CD26,MMP-2,3 and 12,uPA/Urokinase)and 10 PIs(Protease Nexin II,Cystatin B and C,EMMPRIN/CD147,Latexin,Lipocalin-1,Serpin E1,TFPI,TFPI-2,TIMP-1,2 and 4).Healthy and FECD specimens showed similar variation patterns according to morphology for secretion of ADAMTS1,MMP-3 and 12.However,opposing patterns between healthy and FECD populations were observed for Cathepsin B and D.Moreover,some proteins did not show variation according to phenotype in healthy CECs,but did in FECD CECs:Cathepsin A,Cystatin C,TFPI-2 and total TIMPs.For the other proteins,secretion did not vary according to morphology or no specific pattern was distinguishable.Conclusions:To conclude,our results suggest that cell phenotype is linked to the secretion of certain proteases/PIs in both groups.However,there seems to be differences in secretion of particular proteases and PIs between FECD and healthy specimens as morphology did not have a similar influence.These differences might initiate an imbalance between proteases and PIs explaining the irregular thickening of the Descemet membrane seen in FECD.

关 键 词：Fuchs endothelial corneal dystrophy(FECD) CORNEA corneal endothelial cells(CECs) PROTEASE phenotype 

分 类 号：R73[医药卫生—肿瘤]