Three previously undescribed steroidal glycoalkaloids from Solanum lyratum and their anti-tumor activities  

白英中三个未被报道的甾体糖苷生物碱及其抗肿瘤活性

作　　者：Liangyu Liu Yuke Yang Xiao Du Tong Wu Jiannong Wang 刘良裕;杨宇珂;杜肖;吴桐;王建农(中国中医科学院西苑医院基础医学研究所中药药理北京市重点实验室,北京100091;华润三九医药股份有限公司,广东深圳518110;中国中医科学院博士后流动站,北京100700)

机构地区：[1]Key Laboratory of Chinese Medicine Pharmacology,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,100091 Beijing,China [2]China Resources Sanjiu Medical&Pharmaceutical Co.Ltd.,Shenzhen 518110,China [3]Postdoctoral Mobile Research Station of China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2022年第3期192-201,共10页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No.81872989).

摘　　要：In the present study,three previously undescribed steroidal glycoalkaloids(compounds 1–3)were isolated from Solanum lyratum.Their structures were elucidated based on comprehensive spectroscopic data.Their anti-angiogenesis and anti-metastatic activities were evaluated by MTT and wound-healing assays,respectively.Tumor-derived vascular endothelial cells(TdECs),obtained by co-culture of A549 and human umbilical vein endothelial cells(HUVECs),were treated with compounds 1–3.Results showed that compounds 1–3 significantly inhibited the migration of TdECs at 25μM despite the weak cytotoxic activities,which indicated that the compounds exerted anti-tumor activities by inhibiting metastasis,rather than directly inhibiting the proliferation of TdECs.本研究从白英中分离得到3个未被报道的甾体糖苷生物碱(化合物1–3),借助综合光谱分析,阐明了它们的结构。通过MTT和划痕愈合实验分别评价其抗肿瘤血管生成和转移活性,为更好的模拟体内肿瘤环境,将人非小细胞肺癌细胞株A549与人脐静脉内皮细胞共培养获得肿瘤血管内皮细胞(TdECs)模型,用化合物1–3分别处理TdECs。结果显示,尽管它们对TdECs的细胞毒活性相对较弱,但在25μM时均能明显抑制TdECs的迁移。这些结果表明,化合物1–3并不是通过影响肿瘤血管内皮细胞的增殖直接作用于血管生成,而是通过抑制肿瘤细胞的转移发挥抗肿瘤活性。

关 键 词：Solanum lyratum Steroidal glycoalkaloids ANTI-TUMOR METASTASIS ANGIOGENESIS 

分 类 号：R284[医药卫生—中药学]