ORAC法比较几种动物蛋白肽的抗氧化活性  

Comparison of Antioxidant Activity of Several Animal Protein-derived Peptides by ORAC

作　　者：况秀平 黄蓉萍 韩绍聪 张琼谊 闫昌誉 李维熙 Kuang Xiuping;Huang Rongping;Han Shaocong;Zhang Qiongyi;Yan Changyu;Li Weixi(Yunnan University of Traditional Chinese Medicine,College of Traditional Chinese Medicine,Kunming 650500;Ji’nan University,College of Pharmacy,Guangzhou 510632,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]暨南大学药学院,广东广州510632

出　　处：《广东化工》2022年第7期14-15,52,共3页Guangdong Chemical Industry

基　　金：国家自然科学基金(81560661,81960780);云南省基础研究中医联合专项(2017FF116(-015),2019FF002(-007))。

摘　　要：目的:探讨乌鸡肽、酪蛋白肽、鱼皮肽和牛韧带肽4种食源性动物蛋白肽的抗氧化活性。方法:采用氧自由基吸收能力法(ORAC),对4种蛋白肽的总抗氧化能力进行评价研究。结果:乌鸡肽、酪蛋白肽、鱼皮肽和牛韧带肽的ORAC当量分别是4.190、4.999、0.5897和1.686μmol Trolox/mg。结论:4种动物蛋白肽均有一定的抗氧化活性(0.5~5.0μmol Trolox/mg),其中酪蛋白肽活性最强,其次是乌鸡肽、牛韧带肽和鱼皮肽。Objective: To investigate the antioxidant activities of four animal derived protein peptides, including black chicken peptide, casein peptide, fish skin peptide and bovine ligamentum peptide. Methods: The total antioxidant capacity of peptides was evaluated by oxygen free radical absorption capacity(ORAC)method. Results: The ORAC equivalents of black chicken peptide, casein peptide, fish skin peptide and bovine ligamentum peptide were 4.190, 4.999, 0.5897 and 1.686 μmol Trolox/mg respectively. Conclusion: The four peptides have antioxidant capacity(0.5~5.0) μmol Trolox/mg, in which casein peptide has the strongest activity, followed by black chicken peptide, bovine tenascin peptide and fish skin peptide.

关 键 词：ORAC 食源性 动物蛋白肽 抗氧化 TROLOX 

分 类 号：TS201.2[轻工技术与工程—食品科学]