福建大田茶树品种资源生化成分特征分析与评价  

Analysis of biochemical components of tea variety resources in Datian,Fujian

作　　者：陈潇敏 章进汕 金珊[1] 邵淑贤 刘永崇 吴文晞 王鹏杰[1] 赵峰 叶乃兴[1] CHEN Xiao-min;ZHANG Jin-shan;JIN Shan;SHAO Shu-xian;LIU Yong-chong;WUWen-xi;WANG Peng-jie;ZHAO Feng;YE Nai-xing(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Datian Bureau of Agriculture and Rural Affairs,Sanming,Fujian 366100,China;Fujian Hexiyan Agricultural Development Co.,Ltd,Sanming,Fujian 366118,China;Hengzheng Testing Technology Company,Fuzhou 350100,China;School of Pharmacy,Fujian University of Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建农林大学园艺学院,福州350002 [2]大田县农业农村局,福建三明366100 [3]福建省和溪岩农业开发有限公司,福建三明366118 [4]福建恒正检测技术有限公司,福州350100 [5]福建中医药大学药学院,福州350122

出　　处：《南方农业学报》2022年第2期381-390,共10页Journal of Southern Agriculture

基　　金：国家现代农业产业技术体系项目(CARS-19);福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A01);福建农林大学优秀硕士学位论文资助基金项目(1122YS01003);福建中医药大学引进人才科研启动项目(2801/701190097)。

摘　　要：【目的】探明福建省大田县茶树品种资源生化成分特征,为其开发利用和茶树特异品种筛选提供科学依据。【方法】以24份大田茶树品种资源为试材,采用高效液相色谱法(HPLC)测定儿茶素组分,应用超高效液相色谱—质谱联用法(UHPLC-MS/MS)测定嘌呤生物碱组分及氨基酸组分,并进行描述性分析、主成分分析、聚类热图分析及特异资源筛选。【结果】24份大田茶树品种资源的儿茶素总量和表没食子儿茶素-3-O-(3-O-甲基)没食子酸酯(EGCG3"Me)含量分别为60.00~251.75和2.92~22.90 mg/g,其遗传多样性指数分别为2.11和2.06;咖啡碱、苦茶碱和茶氨酸含量分别为3.69~68.71、0.29~48.59和0.28~39.52 mg/g,其遗传多样性指数分别为1.98、1.99和1.96。对主要品质成分进行主成分分析,前3个主成分的累积方差贡献率超过80.00%。基于儿茶素、嘌呤生物碱及氨基酸组分共30个生化成分,可将24份大田茶树品种资源分为3个类群,A类群氨基酸组分含量较高,B类群生化成分含量均表现较低,C类群的儿茶素组分及嘌呤生物碱组分含量较高,不同类群在表没食子儿茶素没食子酸酯(EGCG)、EGCG3"Me、咖啡碱、苦茶碱和茶氨酸含量等主要指标有明显差异。综合分析结果,筛选到16份儿茶素组分特异资源(高儿茶素10份,高EGCG 5份、高EGCG3"Me 1份)、23份嘌呤生物碱组分特异资源(高嘌呤生物碱9份、高苦茶碱13份、高咖啡碱1份)及6份高茶氨酸茶树资源。【结论】大田茶树品种资源均含有EGCG3"Me和苦茶碱,儿茶素、嘌呤生物碱及氨基酸组分具有丰富的遗传多样性;通过系统的鉴定评价,筛选出一批生化成分特异资源,可作为茶树特异性状育种材料。【Objective】To explore the biochemical composition characteristics of tea varieties resources in Datian County,Fujian Province,so as to provide scientific basis for the development and utilization of tea variety resources and the breeding ofspecific tea varieties.【Method】Twenty-fourtea variety resources in Datian were used as test materials to determine the catechin components by HPLC,and the purine alkaloids and amino acid components by UHPLC-MS/MS to conduct descriptive analysis,principal component analysis,cluster thermogram analysis and specific resource screening.【Result】The contents of total catechins and EGCG3"Me in 24 tea variety resources were 60.00-251.75 and 2.92-22.90 mg/g respectively,and theirgenetic diversity indexes were 2.11 and 2.06;the contents of caffeine,theacrine and theanine were3.69-68.71,0.29-48.59 and 0.28-39.52 mg/g,respectively,and their genetic diversity indexes were 1.98,1.99 and 1.96.The principal component analysis of the main quality components showed that the cumulative variance contribution rate of the first 3 principal components exceeded 80.00%.Considering 30 biochemical components based on catechins,purine alkaloids and amino acids 24 tea variety resources could be divided into 3 groups:Group A had higher contents of amino acids;group B had lower contents of biochemical components;and group C had higher contents of catechins and purine alkaloids.Significant differences existed in the main indexes of EGCG,EGCG3"Me,caffeine,theacrine and theanine among different groups.According to comprehensive analysis results,16 catechin component specific resources(10 high catechins,5 high EGCG,1 high EGCG3"Me),23 purine alkaloid component specific resources(9 high purine alkaloids,13 high theacrine,1 high caffeine)and 6 high theanine tea resources were screened.【Conclusion】Tea variety resources in Datian contain EGCG3"Me and theacrine.Catechins,purine alkaloids and amino acid components have rich genetic diversity.Through systematic identification and evaluation,a number of

关 键 词：茶树 品种资源 苦茶碱 表没食子儿茶素-3-O-(3-O-甲基)没食子酸酯(EGCG3"Me) 茶氨酸 福建大田县 

分 类 号：S571.1[农业科学—茶叶生产加工]