低共熔溶剂提取的黄精多糖性质分析  

Properties of Polysaccharides from Polygonatum sibiricum Extracted with Deep Eutectic Solvents

作　　者：刘旭 孟继坤 葛鑫会 张楠[1] 裴海生[2] 张秀清[1] LIU Xu;MENG Jikun;GE Xinhui;ZHANG Nan;PEI Haisheng;ZHANG Xiuqing(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Agricultural Product Processing Engineering Research Institute,Planning and Design Institute of Ministry of Agriculture,Beijing 100121,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]农业部规划设计研究院农产品加工工程研究所,北京100121

出　　处：《食品工业科技》2022年第11期52-57,共6页Science and Technology of Food Industry

基　　金：亚临界电解离子水限制性降解结构多糖作用机制研究项目(21576142)。

摘　　要：为研究低共熔溶剂提取条件对黄精多糖性质及体外抗氧化活性的影响,以鸡头黄精为原料,对不同条件下低共熔溶剂提取得到的黄精多糖进行相对分子量、单糖组成等基本性质和体外抗氧化活性的测定。结果显示,相比于传统水提醇沉法,采用低共熔溶剂法提取黄精多糖,70℃时得率为18%,提高了36%,所得多糖的相对分子量变小且半乳糖含量升高;100℃提取的多糖相对分子量更小,且主要成分为葡萄糖。羟基自由基与DPPH自由基清除率、总抗氧化能力测定结果均表明,采用低共熔熔剂在70℃条件下提取的黄精多糖体外抗氧化能力明显高于传统水提醇沉法和低共熔溶剂法在100℃条件下提取的黄精多糖。当浓度为3.0 mg/mL时,采用低共熔熔剂在70℃提取的黄精多糖总抗氧化能力为4.5 U/mL,是水提多糖的4.3倍,是100℃条件提取黄精多糖的7.4倍。低共熔溶剂对黄精多糖具有降低分子量、提高抗氧化性等效果,研究结果可以为低共熔溶剂在多糖提取方面的应用提供参考。In order to understand the effect of deep eutectic solvents(DESs)extraction conditions on the properties changes and antioxidant activity in vitro of Polygonatum sibiricum polysaccharides(PSP),Polygonatum sibiricum was taken as the main raw material,and the relative molecular weight,monosaccharide composition and antioxidant activities of polysaccharides extracted with DESs were analyzed.The results showed that compared with the traditional water extraction method,the yield of polysaccharides extracted by 70℃DESs was 18%,which was 36%higher than that by traditional water extraction.The molecular weight of PSP decreased and the galactose content increased;the molecular weight of HDPSP which extracted at 100℃was lower,and the main component was glucose.The scavenging rates of hydroxyl free radical and DPPH free radical and the total antioxidant capacity of Polygonatum sibiricum polysaccharide showed that the antioxidant capacity of Polygonatum sibiricum polysaccharide extracted with DESs at 70℃was significantly higher than that of polysaccharide extracted at 100℃and water extraction.When the concentration was 3.0 mg/mL,the total antioxidant capacity of polysaccharides extracted at 70℃was 4.5 U/mL,4.3 times that of water-extracted polysaccharides,and 7.4 times that of polysaccharides extracted at 100℃.Deep eutectic solvent can reduce molecular weight and improve antioxidant activity of Polygonatum sibiricum polysaccharide.The results can provide reference for the application of deep eutectic solvent in polysaccharide extraction.

关 键 词：黄精 多糖 低共熔溶剂 分子量 单糖组成 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]