阿胶活性肽的结构鉴定及活性筛选  

Structure Identification and Activity Screening of Active Peptides in Colla Corii Asini(Ejiao)

作　　者：王莹雪 樊雨梅 廖峰 于志鹏 WANG Yingxue;FAN Yumei;LIAO Feng;YU Zhipeng(College of Food Science and Technology,Bohai Univercity,Jinzhou 121013,China;National Engineering Research Center for Gelatin-based Traditional Chinese Medicine,Dong’e Ejiao Co.Ltd.,Liaocheng 252201,China;School of Food Science and Engineering,Hainan University,Haikou 570228,China)

机构地区：[1]渤海大学食品科学与工程学院,辽宁锦州121013 [2]国家胶类中药工程技术研究中心,东阿阿胶股份有限公司,山东聊城252201 [3]海南大学食品科学与工程学院,海南海口570228

出　　处：《食品科学》2022年第10期207-213,共7页Food Science

基　　金：山东省泰山产业领军人才传统产业创新类项目(TSCY20170233)。

摘　　要：利用液相色谱-串联质谱联用技术鉴定阿胶模拟胃肠道消化产物中肽的结构,并通过分子对接技术从中筛选潜在的降血压、抗老年痴呆和抗肿瘤的活性肽。结果表明,从阿胶消化产物中获得519种活性肽,其分子质量在661.4~2851.4 Da之间,主要为胶原蛋白和血红蛋白的酶解产物;获得最具潜力的降血压肽KGETGLR、抗老年痴呆肽SGLDGAKG和抗肿瘤肽ADGVAGPK;氢键相互作用和静电相互作用在阿胶肽发挥降血压、抗老年痴呆和抗肿瘤活性中都起着重要的作用。In this study,liquid chromatography-tandem mass spectrometry(LC-MS/MS)was used to identify the structures of peptides in Colla Corii Asini(Ejiao)subjected to simulated gastrointestinal digestion.The peptides were screened for their anti-hypertensive,anti-Alzheimer,and anti-tumor potential by molecular docking.The results showed that 519 active peptides with molecular mass ranging from 661.4 to 2851.4 Da were obtained from the digested products of Ejiao.These peptides were mainly derived from the enzymatic hydrolysis of collagen and hemoglobin.Peptides KGETGLR,SGLDGAKG,and ADGVAGPK were found to have the highest anti-hypertensive,anti-Alzheimer and anti-tumor potential.Hydrogen bond and electrostatic interactions played a vital role in the anti-hypertensive,anti-Alzheimer,and anti-tumor activities of the Ejiao peptides.

关 键 词：胃肠道消化 阿胶活性肽 分子对接 降血压 抗老年痴呆 抗肿瘤 

分 类 号：TS201.4[轻工技术与工程—食品科学]