环糊精葡萄糖基转移酶在天然产物糖基化修饰中的应用  

Application of Cyclodextrin Glucosyltransferase in the Glycosylation Modification of Natural Products

作　　者：张国宁 冯婧娴 杨颖博 陈万生 肖莹 ZHANG Guo-ning;FENG Jing-xian;YANG Ying-bo;CHEN Wan-sheng;XIAO Ying(Research and Development Center of Chinese Medicine Resources and Biotechnology,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203;Shanghai Tufeng Pharmaceutical Technology Co.,Ltd.,Shanghai 201203;Medical Guarantee Center,Changzheng Hospital,Naval Medical University,Shanghai 200003)

机构地区：[1]上海中医药大学中药研究所中药资源与生物技术中心,上海201203 [2]上海图锋医药科技有限公司,上海201203 [3]海军军医大学附属长征医院医疗保障中心,上海200003

出　　处：《生物技术通报》2022年第3期246-255,共10页Biotechnology Bulletin

基　　金：国家自然科学基金项目(81874335和31872665);上海市中央引导地方科技发展资金项目(YDZX20203100002948);上海中医药大学预算内项目(2020LK013)。

摘　　要：环糊精葡萄糖基转移酶(cyclodextrin glucosyltransferase,CGTase)是一种可催化淀粉或多糖中α-1,4键断裂并环化形成环糊精(cyclodextrins,CDs)的α-淀粉酶。CGTase在工业上主要用于制造环糊精,近年来利用其转糖基作用改造天然产物的性质取得了令人瞩目的研究进展,正成为CGTase应用颇有前途的发展方向。本文结合CGTase的来源、蛋白结构及催化机制等相关研究,重点介绍近年来CGTase在天然产物糖基化修饰中的应用,为CGTase在该领域的深入研究及应用提供参考。Cyclodextrin glucosyltransferase(CGTase)is a kind ofα-amylase that can catalyze the cleavage and cyclization ofα-1,4 bonds in starch or polysaccharides to form cyclodextrins(CDs).CGTase is mainly used in industry to produce cyclodextrins.In recent years,There has been remarkable progress in the use of the transglycosylation effect of CGTase to modify the properties of natural products,which is becoming a promising development direction.This review introduces the source,protein structure and catalytic mechanism of CGTase,and focuses on its application in glycosylation modification of natural products in recent years,aiming to provide references for the in-depth research and application of CGTase in this field.

关 键 词：环糊精葡萄糖基转移酶 蛋白结构 天然产物 糖基化反应 

分 类 号：Q55[生物学—生物化学]