动态高压微射流处理对低盐肌原纤维蛋白溶解度和结构的影响  

Effect of dynamic high-pressure microfluidization treatment on the solubility and structure of myofibril protein in low-salted system

作　　者：吴佳[1] 赵鸾 魏娜 赵磊 王立宇 夏杨毅[1,2] WU Jia;ZHAO Luan;WEI Na;ZHAO Lei;WANG Liyu;XIA Yangyi(College of Food Science,Southwest University,Chongqing 400700,China;Chongqing Special Food Programme and Technology Research Center,Chongqing 400700,China)

机构地区：[1]西南大学食品科学学院,重庆400700 [2]重庆市特色食品工程技术研究中心,重庆400700

出　　处：《食品与发酵工业》2022年第11期129-135,共7页Food and Fermentation Industries

基　　金：重庆市技术创新与应用发展专项(cstc2020jscx-tpyzxX0010)。

摘　　要：探讨了动态高压微射流(dynamic high pressure microfluidization,DHPM)处理压力(0、34、69、103、138、172 MPa)和处理次数(0~12次)对低盐(0.15 mol/L NaCl)肌原纤维蛋白溶解度和结构的影响。结果表明,随着DHPM处理压力和次数的增加(P<0.05),蛋白溶解度显著增加(P<0.05),但103 MPa后不再显著(P>0.05)、Zeta-粒径显著降低(P<0.05)。蛋白表面疏水性经DHPM处理后显著增加(P<0.05),但其随着压力的增加显著降低(P<0.05),随着次数的增加先增加后降低(P<0.05),这是蛋白结构展开又折叠的结果。经DHPM处理后,激光共聚焦显微镜显示蛋白颗粒变小、尺寸均匀;十二烷基硫酸钠-聚丙烯酰氨凝胶电泳显示蛋白发生了降解;拉曼分析显示α-螺旋含量明显下降,无规则卷曲含量增加。综上,DHPM处理能破碎蛋白,使蛋白解聚、尺寸变小,结构发生改变,进而改善低盐下肌原纤维蛋白的溶解度。This experiment explored the effects of dynamic high-pressure microfluidization(DHPM)treatment pressure(0,34,69,103,138,172 MPa)and treatment times(0-12 times)on the solubility and structure of the myofibrillar protein in low-salted system(0.15 mol/L NaCl).The results showed that as the pressure and times of DHPM treatment increased(P<0.05),the protein solubility increased significantly(P<0.05),but it was no longer significant after the pressure over 103 MPa(P>0.05)and the Zeta-particle size decreased significantly(P<0.05).Moreover,the hydrophobicity of the protein surface increased significantly after DHPM treatment(P<0.05).It decreased significantly with the increase of pressure(P<0.05),and increased first and then decreased with the increase of the number of times(P<0.05).This was the result of the unfolding and folding of the protein structure.After DHPM treatment,confocal laser scanning microscopy(CLSM)showed that the protein particles became smaller and more uniform in size.The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)showed that the protein has been degraded.Raman analysis showed that theα-helix content decreased significantly,while the content of random curls increased.In summary,DHPM treatment could break the protein,depolymerize the protein,reduce the size,and change the structure,thereby improving the solubility of myofibrillary protein under low salt.

关 键 词：动态高压微射流(DHPM) 肌原纤维蛋白 低盐 溶解度 

分 类 号：TS201.21[轻工技术与工程—食品科学]