采用HS-SPME-GC-MS分析蜂蜜中的5-羟甲基糠醛衍生物  

Analysis of Hydroxymethyl Furfural Derivatives in Honeys by HS-SPME-GC-MS

作　　者：陈路芳 李丹丹[1] 倪辉[1,2] 杨远帆 CHEN Lufang;LI Dandan;NI Hui;YANG Yuanfan(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021 [2]厦门市食品生物工程技术研究中心,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2022年第3期221-228,共8页Journal of Jimei University：Natural Science

基　　金：国家自然科学基金项目(31371751)。

摘　　要：以3种原蜜及其加热浓缩蜂蜜为实验材料,利用顶空固相微萃取联动气质色谱(head space solid phase micro-extraction gas chromatography-mass spectrometry,HS-SPME-GC-MS)分析技术对比分析其中的5-羟甲基糠醛衍生物的含量。结果表明,利用HS-SPME-GC-MS方法从蜂蜜样品中鉴定出3种5-羟甲基糠醛衍生物;3种物质在质量浓度范围内均呈良好的线性关系,相关系数r^(2)>0.990;检出限低于3.33×10^(-2)μg/L,定量限低于3.33μg/L,加标回收率在80.76%~115.5%。研究发现,糠醛在3种加热浓缩蜂蜜中的含量显著高于对应的3种原蜜,而3-甲基-2-戊酮及2-乙基-1-己醇含量在3种加热浓缩蜂蜜与对应原蜜之间没有显著差异。说明,采用HS-SPME-GC-MS法测定蜂蜜中的糠醛含量可以区分原蜜及加热浓缩的蜂蜜。In this study,three kinds of raw honey,as well as their heated and concentrated products were used as experimental materials to analyze the content of 5-hydroxymethylfurfural(5-HMF)derivatives by head space solid phase micro-extraction gas chromatography-mass spectrometry(HS-SPME-GC-MS).The results showed that three 5-HMF derivatives were identified.All three derivatives showed a good linear relationship in the range of mass concentration,and the correlation coefficient is r^(2)>0.990.The detection limit was lower than 3.33×10^(-2) μg/L,the quantification limit was lower than 3.33μg/L,and the recovery rate were in the range of 80.76%-115.5%.The furfural content in three heated and concentrated honey was significantly higher than that in raw honey,while the contents of 3-methyl-2-pentanone and 2-ethyl-1-hexanol showed no significant difference between the concentrated honey and raw honey.This study indicated that determination of furfural content in honey by the HS-SPME-GC-MS method is able to distinguish raw honey from heated concentrated honey.

关 键 词：5-羟甲基糠醛衍生物 蜂蜜 顶空固相微萃取 气质色谱 

分 类 号：S896.8[农业科学—特种经济动物饲养]