基于响应面法的(鱼安)(鱼康)活性肽制备工艺  

Optimization of monkfish active peptide using response surface methodology

作　　者：王雪芹[1,2] 滕李超 于华华 邢荣娥[1,2] 李鹏程 WANG Xue-qin;TENG Li-chao;YU Hua-hua;XING Rong-e;LI Peng-cheng(CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室海洋大科学中心,山东青岛266071 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266237 [3]中国科学院大学,北京100049

出　　处：《海洋科学》2022年第5期42-53,共12页Marine Sciences

基　　金：2020年度省新旧动能转换重大工程重大课题攻关项目(2020-1220);中国科学院STS项目(2021T3038)。

摘　　要：(鱼安)(鱼康)是一种低脂肪高蛋白的优质海洋生物资源,其氨基酸含量丰富,蛋白质含量高达75.30%。本实验选用胰蛋白酶,胃蛋白酶,木瓜蛋白酶和菠萝蛋白酶水解(鱼安)(鱼康)鱼糜,比较各酶解产物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除率,确定木瓜蛋白酶为最佳用酶。在单因素实验基础上进行响应面优化实验,确定酶解反应的最佳条件为:加酶量829.51 U/g, pH值7.37,酶解温度47.09℃,酶解时间5.86 h,液固比5.79∶1。在最优条件下验证其DPPH自由基清除率为80.22%±0.63%,接近预测值80.55%。经响应面法实验制备的(鱼安)(鱼康)活性肽粉末呈淡黄色,具有较好的抗氧化活性,其羟自由基清除能力的IC_(50)值为1.69 mg/mL,可作为潜在的抗氧化剂。Minced monkfish fillet is an excellent marine biological resource with a diverse range of amino acids,a high protein content of up to 75.30%,and low fat content.In this experiment,four kinds of proteases,i.e.,trypsin,pepsin,papain,and bromelain,were used for hydrolysis to produce the monkfish protein hydrolysate.Papain was chosen as the best enzyme of the four after considering the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging activity of various enzymatic hydrolysates.Based on a single factor experiment,the study used response surface methodology to obtain the optimal conditions.The parameters of the experiment were as follows:enzyme concentration of 829.51 U/g,pH of 7.37,hydrolysis temperature of 47.09℃,hydrolysis time of 5.586 h,and liquidto-solid ratio of 5.79:1.The investigation found that the maximum DPPH scavenging activity was 80.22%±0.63%,which was comparable to the experimental predicted value of 80.55%.The monkfish peptide prepared using response surface methodology was a light-yellow powder with high antioxidant activity.Its IC_(50) of hydroxyl radical scavenging ability was 1.69 mg/mL,showing its potential as an antioxidant.

关 键 词：(鱼安)(鱼康) 酶解反应 木瓜蛋白酶 DPPH自由基清除率 响应面分析 

分 类 号：P745[天文地球—海洋生物学]