响应面法优化微波辅助酶法提取香菇柄可溶性膳食纤维工艺  

Optimization of Microwave-assisted Enzymatic Extraction of Dietary Fiber from Lentinus edods Root by Response Surface Analysis

作　　者：吴丽萍[1] 蔡永久 徐江 胡长玉[1] WU Liping;CAI Yongjiu;XU Jiang;HU Changyu(College of Life and Environmental Sciences,Huangshan University,Huangshan 245000,China;Huangshan Huizhen Food Co.LTD,Huangshan 245000,China)

机构地区：[1]黄山学院生命与环境科学学院,安徽黄山245000 [2]黄山市徽珍食品有限公司,安徽黄山245000

出　　处：《安徽农学通报》2022年第10期125-129,共5页Anhui Agricultural Science Bulletin

基　　金：黄山学院校地合作项目(xdhz202005);黄山市科技计划项目(2020KN-08);横向课题(hxkt2021134,hxkt2021048)。

摘　　要：以废弃物香菇柄为原料,采用微波辅助酶法提取香菇柄可溶性膳食纤维(Soluble Dietary Fiber,SDF)。通过单因素试验探讨微波功率、微波时间、酶解时间、复合酶添加量对SDF提取率的影响;选取对SDF提取率影响较大的因素进行Box-Behnken响应面优化分析并确定最佳工艺参数。结果表明,在微波功率400W、微波时间4min、酶解时间1.3h、复合酶添加量0.5%条件下,SDF的提取率达到14.22%,与模型理论预测值基本相符,该研究可为废弃物香菇柄的综合利用及SDF的提取提供科学依据。The Soluble Dietary Fiber(SDF)was extracted from waste lentinus root by microwave-assisted enzymatic extraction.The effects of microwave power,microwave time,enzyme hydrolysis time and compound enzyme addition on SDF extraction rate were investigated by single factor experiment.The factors that have great influence on SDF extraction rate was selected to carried out Box-behnken response surface optimization and determine the optimum process parameters.The results showed that under the conditions of microwave power 400W,microwave time 4 min,enzymatic hydrolysis time 1.3h and compound enzyme addition 0.5%,the extraction rate of SDF reached 14.22%,which was basically consistent with the theoretical predicted value of the model.This study could provide a theoretical basis for the comprehensive utilization of waste Lentinus edods root and the extraction of SDF.

关 键 词：响应面法 微波 酶 香菇柄 水溶性膳食纤维 

分 类 号：TS201.2[轻工技术与工程—食品科学]