Therapeutic effects of salidroside vs pyrrolidine dithiocarbamate against severe acute pancreatitis in rats  

作　　者：QIAN Jing WANG Xiaohong WEI Benzhong ZHOU Guoxiong ZHU Shunxing LIU Chun 

机构地区：[1]Department of General Surgery,Yizheng Hospital of Nanjing Drum Tower Hospital Group,Yizheng 211900,China [2]Department of Gastroenterology,Yizheng Hospital of Nanjing Drum Tower Hospital Group,Yizheng 211900,China [3]Department of Anesthesiology,Yizheng Hospital of Nanjing Drum Tower Hospital Group,Yizheng 211900,China [4]Department of Gastroenterology,Affiliated Hospital of Nantong University,Nantong 226001,China [5]Department of Laboratory Animal Center,Nantong University,Nantong 226001,China

出　　处：《Journal of Traditional Chinese Medicine》2022年第1期49-57,共9页中医杂志（英文版）

基　　金：Supported by the Traditional Chinese Medicine Technology Development Plan Project of Jiangsu Province in 2020:Basic and Clinical Study on Salidroside in the Adjuvant Treatment of Severe Acute Pancreatitis(No.YB2020088);the Health Innovation Project of Lvyang Jinfeng Plan of Yangzhou City in 2020:Basic and Clinical Study on Salidroside in the Adjuvant Treatment of Severe Acute Pancreatitis(No.LJ202037)。

摘　　要：OBJECTIVE:To evaluate the therapeutic effectiveness of salidroside(Sal)and pyrrolidine dithiocarbamate(PDTC)against severe acute pancreatitis(SAP)in a rat model.METHODS:Rat models of SAP were established by retrograde infusion of sodium taurocholate solution.SAP rats were randomly divided into 6 groups:SAP 3 h group,SAP 24 h group,low-dose Sal treatment group(Sal L+S),middle-dose Sal treatment group(Sal M+S),high-dose Sal treatment group(Sal H+S)and PDTC treatment group(PDTC+S).The serum amylase,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-10(IL-10)levels were determined by optical turbidimetry and enzyme-linked immunosorbent assay.The expression of Beclin-1,microtubule-associated protein light chain 3Ⅱ(LC3Ⅱ),lysosome associated membrane protein 2(LAMP2),interleukin-1 receptor associated kinase 1(IRAK1),inhibitorαof nuclear transcription factor-κB(IκBα),nuclear transcription factor-κB 65(p65)in the pancreas tissues were detected by quantitative real-time polymerase chain reaction and Western blot,while the pIκBαand p-p65 levels were detected by Western blot.Pathological changes of the pancreas and all the other indexes were observed at 3 and 24 h after operation.RESULTS:The serum IL-10 level,IκBαand LAMP2 levels in Sal M+S,Sal H+S and PDTC+S groups were higher than those in SAP 24 h group,while all the other indexes in these three groups were all lower significantly than those in SAP 24 h group.There was no significant difference in all indexes between Sal H+S and PDTC+S groups.CONCLUSION:High-dose Sal has an effectively therapeutic effect on SAP in rats,which was similar to PDTC.

关 键 词：rhodioloside PYRROLIDINES ethylenebis(dithiocarbamates) PANCREATITIS AUTOPHAGY NF-kappa B signal transduction 

分 类 号：R576[医药卫生—消化系统]