基于Illunima Miseq测序技术的黄酒麦曲真菌多样性分析  

Fungal Diversity Analysis of Wheat Qu in Yellow Wine Based on Illunima Miseq Sequencing Technology

作　　者：席啦 孔祥聪 杨少勇 刘忠军 郭壮[1,2] 赵慧君 XI La;KONG Xiang-cong;YANG Shao-yong;LIU Zhong-jun;GUO Zhuang;ZHAO Hui-jun(Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,Hubei,China;Xiangyang Liquor Brewing Biotechnology and Application Enterprise-University Joint Innovation Center,Xiangyang 441053,Hubei,China;Hubei Guxiangyang Wine Industry Co.,Ltd.,Xiangyang 441100,Hubei,China)

机构地区：[1]湖北文理学院湖北省食品配料工程技术研究中心,湖北襄阳441053 [2]襄阳市酿酒生物技术与应用企校联合创新中心,湖北襄阳441053 [3]湖北古襄阳酒业有限公司,湖北襄阳441100

出　　处：《食品研究与开发》2022年第12期185-190,共6页Food Research and Development

基　　金：襄阳市成果转化领域重点科技计划项目(2020AAT00469);百校联百县-高校服务乡村振兴科技支撑行动计划。

摘　　要：该研究以采集的4个黄酒麦曲样品为研究对象,利用Illunima Miseq高通量测序技术对麦曲样品中真菌的群落结构和多样性进行解析。研究发现,Ascomycota(子囊菌门)是麦曲中的主要菌门,平均相对含量高达90.21%;曲霉属(Aspergillus)、酵母属(Saccharomyces)、横梗霉属(Lichtheimia)、根霉属(Rhizopus)、犁头霉属(Absidia)和脉孢菌属(Neurospora)为麦曲中的优势细菌属,其中曲霉菌属(Aspergillus)的平均相对含量高达68.21%。在操作分类单元(operational taxonomic unit,OTU)水平上,OTU3213、OTU3344、OTU4507、OTU623和OTU3471为麦曲中的优势OTU,其中属于Aspergillus的OTU3213平均相对含量高达64.14%。由此可知,黄酒麦曲中含有大量的核心真菌微生物。In this study,four wheat starter samples used for yellow wine production were collected and the fungal community structure and diversity were analyzed by high-throughput sequencing using the Illunima Miseq platform. The results showed that Ascomycota was the dominant fungal phylum in the wheat starters,with an average relative abundance of up to 90.21%. Moreover,Aspergillus,Saccharomyces,Lichtheimia,Rhizopus,Absidia,and Neurospora were the dominant fungal genera in the wheat starters. The average relative abundance of Aspergillus was as high as 68.21%. At the operational taxonomic unit(OTU)level,OTU3213,OTU3344,OTU4507,OTU623,and OTU3471 were the dominant OTUs. OTU3213,which is affiliated with Aspergillus,had an average relative abundance of up to 64.14%. Thus,it can be concluded that the wheat starters used for yellow wine production contained a large number of core fungal microorganisms.

关 键 词：麦曲 Illunima Miseq高通量测序 真菌 群落结构 多样性 

分 类 号：TS262.4[轻工技术与工程—发酵工程]